1 0 0 0 OA 車軸藻脱膜モデルによる原形質流動の研究
- 著者
- 新免 輝男 富永 義人 田沢 仁
- 出版者
- 一般社団法人 日本生物物理学会
- 雑誌
- 生物物理 (ISSN:05824052)
- 巻号頁・発行日
- vol.25, no.1, pp.11-16, 1985-01-25 (Released:2009-05-25)
- 参考文献数
- 32
Two types of demembranated cell models have been established using Characeae internodal cells; the tonoplast-free cell model and the plasmalemma-permeabilized cell model. In these models, both ATP and Mg2+ were essential for the maintenance of cytoplasmic streaming. Ca2+ inhibited the streaming in both models. However, the Ca2+-sensitivity of the streaming in the plasmalemma-permeabilized model was 1000 times higher than the tonoplast-free model, suggesting that some Ca2+-sensitizing component was dispersed or disorganized by the disintegration of the tonoplast. Using the actin bundles present in the tonoplast-free cells, the streaming was reconstituted with the endoplasm from other Characeae cells or the rabbit skeletal muscle, scallop or Physarum myosin. Ca2+ accelearated the streaming reconstituted with scallop myosin but inhibited that reconstituted with Physarum myosin. These opposite effects of Ca2+ on reconstituted streamings agree with the effects of Ca2+ on the respective actomyosin ATPases.
1 0 0 0 OA 車軸藻細胞およびイカ巨大神経線維の膜特性の比較研究
- 著者
- 田沢 仁 新免 輝男 山岸 俊一
- 出版者
- 一般社団法人 日本生物物理学会
- 雑誌
- 生物物理 (ISSN:05824052)
- 巻号頁・発行日
- vol.23, no.2, pp.47-57, 1983-03-25 (Released:2009-05-25)
- 参考文献数
- 71