著者

杉浦 衛 小木 曾太郎 陳 方祥 岩田 忠彦

出版者

公益社団法人日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.89, no.10, pp.1440-1445, 1969-10-25

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Separative determination of α-chymotrypsin and Bromelain, frequently used as an anti -inflammatory enzyme preparation, was examined. p-Chloromercuribenzoic acid (PCMB) was used as the inhibitor of Bromelain. Since Bromelain activity in preparations was completely inhibited by 10^<-4>_M of PCMB in the reaction system, remaining α-chymotrypsin activity was determined with good accuracy. The difference between their total activity and α-chymotrypsin activity agreed with Bromelain activity.