著者

小泉 稔 堀口 幸太郎 冨田 陽子 KATO Yukio HARIGAYA Toshio

出版者

日本繁殖生物学会

雑誌

家畜繁殖研究會誌 (ISSN:09168818)

巻号頁・発行日

vol.49, no.6, pp.465-472, 2003

被引用文献数

1 7

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In this study, we attempted to examine the presence of prolactin (PRL) messenger ribonucleic acid (mRNA) and protein in the mouse nipple and mammary gland in pregnancy and lactation. PRL-like substances were found by immunohistochemistry using an antibody against the mouse PRL (mPRL) in the sebaceous gland cells of the nipple during late pregnancy and lactation, and the cistern of alveoli in mammary glands during lactation. Western blot analysis of proteins extracted from the nipple and the mammary gland showed immunoreactive bands corresponding to molecular weights of approximate 16 kDa and 32 kDa, respectively. The expression of mRNA for mPRL in the nipple and mammary gland during late pregnancy and lactation was demonstrated by reverse transcription-polymerase chain reaction (RT-PCR), Southern blotting, and nucleotide sequence analyses. These results suggest that mPRL mRNA and its translation product are synthesized in the mouse nipple.

著者

米原 三揮 半田 岳志 松鹿 弥生 小泉 稔 富山 友恵

出版者

一般社団法人 電気学会

雑誌

電気学会論文誌C(電子・情報・システム部門誌) (ISSN:03854221)

巻号頁・発行日

vol.140, no.3, pp.398-408, 2020-03-01 (Released:2020-03-01)

参考文献数

15

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The demand for navigation is rising as railway transportation services is becoming complex. Real-time data integration from multiple business systems is required for passenger-oriented navigation. Conventional mediators, real-time data integration technique which converts queries and data between business systems and applications based on their each schemes and mapping between them, can not follow expansion both of applications and business systems. In this paper, we propose a new mediator to solve the scalability problem with core-global scheme, which allows independednt mappings with each schemes of them and expresses principal components of business systems and applications. The 2 features of core-global scheme reduce effects of scheme changes on other mappings so that the mediator has scalability. Furthermore, we propose an architecture of the mediator that reduces effectivity loss of queries. We implemented a prototype and evaluated its scalability and query effectivity for an application of railway navigation.