著者

松原 由美 上農 正剛 赤木 保吉

出版者

九州保健福祉大学QOL研究機構社会福祉学研究所

雑誌

QOL研究機構社会福祉学研究所研究・活動報告 (ISSN:24342610)

巻号頁・発行日

no.2018, pp.21-30, 2019-03-31

著者

松原 由美子

出版者

一般社団法人 日本血栓止血学会

雑誌

日本血栓止血学会誌 (ISSN:09157441)

巻号頁・発行日

vol.31, no.4, pp.432-438, 2020 (Released:2020-08-12)

参考文献数

13

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Platelets are released from mature megakaryocytes (MKs) and are used for therapeutic application, especially platelet transfusion. Platelet concentrates used for platelet transfusions are currently supplied by volunteer donors. Due to their short shelf life (4 days in Japan and 5 days in US), however, donor-dependent platelet concentrates are associated with practical problems, such as the limited supply and the risk of infection. Thus, new strategies for manufacturing MKs and subsequently platelets from a donor-independent source are urgently needed. Mesenchymal stem cells (MSCs) are known to be non-hematopoietic multipotent progenitor-cells. We found that MSCs/stromal cells differentiated into MKs and platelets. The clinical needs for platelet transfusions are increasing. Adipose tissue-derived MSCs/stromal cells are an attractive candidate cell source because inducing these cells into MK-lineages requires no gene transfer and only endogenous transcription factors containing p45NF-E2/Maf, an MK-inducing factor and endogenous thrombopoietin, a primary cytokine that drives MK lineages. Thus, we developed a manufacturing system for platelets from donor-independent cell source, human adipose-derived mesenchymal stromal/stem cell line (ASCL). ASCL satisfied the minimal criteria for defining MSC by The International Society for Cellular Therapy. ASCL-derived platelets (ASCL-PLT) were obtained with a peak at Day 12 of culture using MK lineage induction media. We observed that CD42b-positive cells expressed a MSC marker CD90 in relation to cell adhesion. The pattern of in vivo kinetics after being infused into irradiated immunodeficient NSG mice was similar to that of platelet concentrates. ASCL-PLT has characterization observed in other platelet populations and might have additional function as MSC. The present protocol is a simple method that requires no feeder cells, further enhancing the clinical application of our approach.

著者

松原 由美

出版者

明治安田生活福祉研究所

雑誌

生活福祉研究 : 明治安田生活福祉研究所調査報

巻号頁・発行日

pp.116-127, 2011-12

著者

松原 由美

出版者

九州保健福祉大学

雑誌

九州保健福祉大学研究紀要 (ISSN:13455451)

巻号頁・発行日

no.12, pp.79-84, 2011-03

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The human life-span is increasing worldwide. During the 1970s, senior citizens comprised 7.1% of the population in Japan, and by 1994, they constituted 14% of the population. Life expectancy has risen to 79.19 years for men and 85.99 years for women. This extended lifespan of seniors brings with it an increased need for improvement in quality of life. One intervention that can be used to meet this need is long-term music therapy. The combination of singing and listening to "familiar music"or"reminiscent music"while utilizing social activity was explored in the context of an evidence- based medicine framework. A deep understanding of the elderly is important in order to determine the effects of the music therapy and their relationship with the life history of the patient.