著者

道券 一浩 桧垣 恵 東海林 洋子 嶋田 甚五郎 西岡 久寿樹 水島 裕

出版者

日本DDS学会

雑誌

Drug Delivery System (ISSN:09135006)

巻号頁・発行日

vol.8, no.4, pp.257-261, 1993-07-10 (Released:2009-02-23)

参考文献数

14

被引用文献数

1 1

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Antisense oligonucleotide hold great promise as potential therapeutic agents for inhibiting the expression of undesirable genes specifically. Whereas, interleukin-1β(IL-1β) play a key role in the inflammation and the developing of rheumatoid arthritis(RA). To block the IL-1β production by antisense DNA method, we synthesized antisense oligonucleot ides (20mers)with ph osphoroth ioate linkages targeting the human IL-1β mRNA. These antisense oligonucleotides were tested for inhibition of IL-1β production in LPS-stimulated human peripheral blood mononuclear cells from healthy volunteer and primary synobiocytes from patients with RA. These cells were cultured with each oligonucleotide and total IL-1β contents were measured by using ELISA system. Antisense oligonucleotides inhibited the production of IL-1β in both cells in a dose-dependent manner.