著者

武森 信曉

出版者

一般社団法人 日本生物物理学会

雑誌

生物物理 (ISSN:05824052)

巻号頁・発行日

vol.56, no.2, pp.116-119, 2016 (Released:2016-03-31)

参考文献数

10

著者

武森 信曉

出版者

日本電気泳動学会

雑誌

電気泳動 (ISSN:21892628)

巻号頁・発行日

vol.66, no.1, pp.53-57, 2022 (Released:2022-10-08)

参考文献数

7

---

Top-down proteomics, the analysis via mass spectrometry of cellular protein components as intact species without enzymatic digestion, is emerging as a powerful analytical approach to reveal entire proteoform profiles in vivo. To improve the number of proteoforms detected in top-down proteomics, high-resolution proteome fractionation prior to mass spectrometry is crucial, and interest in polyacrylamide gel electrophoresis as a sample pre-fractionation tool has been growing in recent years. This review describes a gel-based proteome fractionation workflow, which facilitates highly efficient protein extraction following polyacrylamide gel electrophoresis, and its application to sample pre-fractionation for top-down proteomics.