- 著者
- 矢島 治明 小川 博 藤井 信孝 船越 奨
- 出版者
- The Pharmaceutical Society of Japan
- 雑誌
- Chemical and Pharmaceutical Bulletin (ISSN:00092363)
- 巻号頁・発行日
- vol.25, no.4, pp.740-747, 1977-04-25 (Released:2008-03-31)
- 被引用文献数
- 12 21
Among various dilute sulfonic acids tested, 2 to 4N ethanesulfonic acid in acetic acid or methylenechloride was found suitable as a deprotecting reagent for the acid labile α-amino protecting groups, such as Boc and Z(OMe), which are currently removed by trifluoroacetic acid or dilute hydrochloric acid, since the deprotection occured selectively within 60 minutes at room temperature leaving intact other side chain protecting groups, such as Z, benzyl ester, S-p-methoxybenzyl and NG-p-methoxybenzenesulfonyl groups. This reagent was applied to the synthesis of three model peptides ; Met and Leu-enkephalin and endorphin.
- 著者
- 矢島 治明 木曽 良明 小川 宏 藤井 信孝 入江 寛
- 出版者
- The Pharmaceutical Society of Japan
- 雑誌
- Chemical and Pharmaceutical Bulletin (ISSN:00092363)
- 巻号頁・発行日
- vol.23, no.5, pp.1164-1166, 1975-05-25 (Released:2008-03-31)
- 被引用文献数
- 27 37
Acidolytic cleavage of various protecting groups currently employed in peptide chemistry by fluorosulphonic and methanesulphonic acids was examined.
- 著者
- 矢島 治明 藤井 信孝
- 出版者
- The Pharmaceutical Society of Japan
- 雑誌
- Chemical and Pharmaceutical Bulletin (ISSN:00092363)
- 巻号頁・発行日
- vol.29, no.2, pp.600-602, 1981-02-25 (Released:2008-03-31)
- 参考文献数
- 16
- 被引用文献数
- 1
Improved chemical synthesis of bovine pancreatic ribonuclease (RNase) A was achieved by applying a new deprotecting procedure with trifluoromethanesulfonic acidthioanisole in combination with a modified air oxidation procedure with glutathione for the disulfide formation. After purifications by affinity chromatography followed by ion-exchange chromatography, a protein with the full enzymatic activity was obtained and subsequently crystallized from 95% ethanol according to Kunitz. A totally synthetic enzyme with full RNase A activity was thus obtained in a crystalline form for the first time.