著者
磯野 良介 大坂 綾太
出版者
日本環境毒性学会
雑誌
環境毒性学会誌 (ISSN:13440667)
巻号頁・発行日
vol.23, no.1, pp.1-9, 2020-07-14 (Released:2020-07-11)
参考文献数
15

Effluent standards in marine areas need to be evaluated based on toxicity values for multiple marine species. We examined the toxicity test method for the Lysmata vittata, which is considered to be sensitive to chemicals. As the shrimp exhibit hermaphroditism, any two individuals could produce a new generation. Wild adult shrimp were collected in 2016 and 2018. Each group was maintained in a 100 L polycarbonate tank under the natural photoperiod for approximately two years. Natural seawater was supplied to the tank through sand-filtration. Artificial shrimp (F1) were obtained from the wild shrimp (2016 group) and were reared to adults under conditions of 25°C and the natural photoperiod in 2018. The F1 spawned at 92 days after hatching (F2). At intervals of about 10 days, nearly 20 adults of the 2016 group and the F1 spawned 1,000 to 7,000 larvae per day at 21–26°C. The larvae from the 2016 group and F1, within one day after hatching, were exposed to hexavalent chromium for 24 h to evaluate the acute toxicity. The 24h-LC50 for zoea of Lysmata vittata closely ranged from 13 to 16 mg/L (nominal concentration) in both toxicity tests. Since these values were lower than previously reported values, Lysmata vittata was sensitive to hexavalent chromium.