著者

清水 一広 川添 祥一 山田 修 長瀬 英生

出版者

公益社団法人 日本薬学会

雑誌

YAKUGAKU ZASSHI (ISSN:00316903)

巻号頁・発行日

vol.105, no.8, pp.784-790, 1985-08-25 (Released:2008-05-30)

参考文献数

22

被引用文献数

1

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The present study was undertaken to determine if adenosine triphosphate-magnesium chloride (ATP-MgCl2) would have beneficial effects on the membrane electrolytes transport and deformability of shocked red blood cells (RBC) with impaired cellular energy metabolism. Shocked RBC suspensions were obtained by incubation of 10% rabbit RBC suspension in phosphate buffered saline at 37°C for 14 h. Cellular ATP level was reduced to approximately 6% of normal value by this incubation. ATP-MgCl2 (100 μM), added to the incubation medium along with glucose (10 mM) after 14 h of incubation, significantly prevented further decreases in cellular ATP and 2, 3-diphosphoglycerate (2, 3-DPG) levels. Increases in intracellular sodium concentration and potassium leakage from RBC were counteracted by simultaneous ATP-MgCl2 and glucose treatment and pH of the incubation medium showed significant reduction compared with the control. It was also observed that glucose utility and deformability of shocked RBC were improved by ATP-MgCl2 and glucose treatment. These results indicate that ATP-MgCl2 would be helpful to restore the impaired RBC function by improving the intracellular energy metabolism.