著者

松井 等 松井 等 高井 章 柏柳 誠

出版者

PHYSIOLOGICAL SOCIETY OF JAPAN

雑誌

日本生理学会大会発表要旨集

巻号頁・発行日

pp.S123, 2005 (Released:2005-04-16)

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In olfactory receptor cells, it is well established that cAMP acts as a main second messenger during odor responses. Biochemical experiments, however, showed failure of accumulation of cAMP in olfactory cilia by odorants of 40% examined (Sklar et al., 1996). We have shown that application of water soluble odorants and volatile odorants induced inward currents in olfactory cells of the Xenopus water nose but dialysis with cAMP did not (Iida and Kashiwayanagi, 1999). Breer and Boekhoff showed that odorants, which did not induce cAMP accumulation in olfactory cilia, induced IP3 accumulation (1990). Dialysis of olfactory cells in Xenopus water nose with IP3 induced inward currents. In addition, the turtle olfactory cells responded to dialysis with cyclic ADP-ribose (cADPR) with an inward current (Sekimoto and Kashiwayanagi, 2003). The magnitudes of the inward current responses to cAMP-increasing odorants were greatly reduced by prior dialyses of a high concentration of cADPR or 8-Br-cADPR, an antagonist. It is possible that IP3 and cADPR play as second messengers during the olfactory transduction. At present, channel molecules which are activated by these second messenger candidates have not been identified in olfactory cells. Transient receptor potential (TRP) channels, which are expressed in many tissues and cell types in vertebrates, are activated by various stimulations. In the present study, we explore roles of TRP channels in the olfactory system by RT-PCR and immunohistochemical techniques. [Jpn J Physiol 55 Suppl:S123 (2005)]

著者

ファラマル アシュリー 日高 弘義 高井 章 富田 忠雄

出版者

Japan Society of Smooth Muscle Research

雑誌

日本平滑筋学会雑誌 (ISSN:03743527)

巻号頁・発行日

vol.21, no.1, pp.57-69, 1985

被引用文献数

4

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Aschoori, F., Hidaka, H., Takai, A. & Tomita, T. <I>Contraction of smooth muscle in Ca-free solution</I>. Japanese Journal of Smooth Muscle Research, 1985, 21 (1), 57-69.-The tonic contractions which are extremely resistant to removal of the external Ca were investigated in the rat was deferens and myometrium. Both the noradrenaline response in the was deferens and the oxytocin response in the myometrium could be repeatedly produced without appreciable diminution in Ca-free solution for more than 24hrs. On the other hand, the tissue Ca content decreased exponentially after Ca-removal with a half time of 130-180min. When Ca was readmitted, no indication of the early transient contraction was observed in the subsequent response in Ca-free solution, but the response was reduced compared with the response before Ca readmission. Verapamil suppressed the response in the presence of Ca, while it had very weak inhibitory effect even at 10μM. Calmodulin antagonists of phenothi-azine derivatives had a strong inhibitory effect on Ca-induced contractions, whereas they had very weak effects on the receptor-mediated contraction in Ca-free solution. Another calmodulin antagonist, W-7 suppressed both Ca-induced contraction and the contractions independent of external Ca. HA-1004, a vasodilator which has a struc-ture similar to W-7, reduced the receptor-mediated contraction in Ca-free solution without much effect on Ca-induced contractions. These results may suggest that the receptor-mediated contractions resistant to Ca-removal are caused by some process without a contribution of the Ca-calmodulin system.