著者

Kosuke Izumitsu Kanako Hatoh Takuya Sumita Yuki Kitade Atsushi Morita Chihiro Tanaka Abdul Gafur Akira Ohta Masataka Kawai Takashi Yamanaka Hitoshi Neda Yuko Ota

出版者

The Mycological Society of Japan

雑誌

Mycoscience (ISSN:13403540)

巻号頁・発行日

vol.53, no.5, pp.396-401, 2012 (Released:2023-03-31)

被引用文献数

106

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We have optimized a simple and rapid preparation procedure for mushroom DNA extraction from colonies on media or from fruiting bodies for PCR amplification. The protocol combines microwaving twice for 1 min, cooling for 10 min, and centrifuging for 5 min. By using this procedure, more than 100 samples of mushroom DNA can be prepared within 1 h. The DNA obtained can be used for (1) identifying mushroom species by PCR and subsequent sequencing, (2) amplifying low copy number genes (at least 2,000 bp), and (3) screening genetic transformants. This technique will contribute to the mycology of mushroom species.