著者

Kosuke Izumitsu Kanako Hatoh Takuya Sumita Yuki Kitade Atsushi Morita Chihiro Tanaka Abdul Gafur Akira Ohta Masataka Kawai Takashi Yamanaka Hitoshi Neda Yuko Ota

出版者

The Mycological Society of Japan

雑誌

Mycoscience (ISSN:13403540)

巻号頁・発行日

vol.53, no.5, pp.396-401, 2012 (Released:2023-03-31)

被引用文献数

106

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We have optimized a simple and rapid preparation procedure for mushroom DNA extraction from colonies on media or from fruiting bodies for PCR amplification. The protocol combines microwaving twice for 1 min, cooling for 10 min, and centrifuging for 5 min. By using this procedure, more than 100 samples of mushroom DNA can be prepared within 1 h. The DNA obtained can be used for (1) identifying mushroom species by PCR and subsequent sequencing, (2) amplifying low copy number genes (at least 2,000 bp), and (3) screening genetic transformants. This technique will contribute to the mycology of mushroom species.

著者

Hidetaka Umata Yuko Ota Masayoshi Yamada Yuka Watanabe Stephan W. Gale

出版者

The Mycological Society of Japan

雑誌

Mycoscience (ISSN:13403540)

巻号頁・発行日

vol.54, no.5, pp.343-352, 2013 (Released:2023-03-07)

被引用文献数

22

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We examined the level of specificity exhibited by seeds of Cyrtosia septentrionalis for germination-inducing fungi. Three wood-decomposing Armillaria species (A. gallica, A. mellea subsp. nipponica and A. tabescens) known to colonize adult plants and an unidentified species of Polyporales isolated from naturally growing protocorms were tested. Xylobolus annosus, a free-living decomposer of Russulales, was included as a control. Seed germination occurred in a sawdust-based medium in sealed and unsealed containers in the presence of all fungi, indicating low mycorrhizal specificity in germination. Moreover, germination occurred even in modified containers in which the fungus was physically isolated from the orchid seeds, indicating that direct seed-fungus contact is not required. Higher germination percentages were observed in sealed containers in which a modified atmosphere, consisting of a lower O2 and a higher CO2 concentration, had been established in the air above the inoculated medium as a result of the saprophytic activity of the fungus. In nature, atmospheric conditions more effective for seed germination might be established by the action of mycobionts in decomposing wood. Seeds germinated in the presence of the unknown Polyporales species underwent further growth and development, as compared with the other fungi tested, when covered with soil.

著者

太田 祐子 Yuko Ota 独立行政法大森林総合研究所 Forestry and Forest Products Research Institute

雑誌

樹木医学研究 (ISSN:13440268)

巻号頁・発行日

vol.10, no.1, pp.3-10, 2006-03-31

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ナラタケ属菌は亜寒帯から亜熱帯にいたる世界中の森林に広く分布する菌であり,広葉樹および針葉樹に重大な根株腐朽病害をおこす病原菌を含むことが知られている.最近広く用いられるようになった分子生物学的手法によって,ナラタケ属菌の種間の系統関係や生態学的研究に新たな知見が得られた.本稿では,ナラタケ属菌の分類,系統関係,生態およびならたけ病の防除について概説する.また防除に関する話題として,近年緑地などでマルチ用資材として利用されている木材チップとナラタケ属菌について最近の研究を紹介する.