- 著者
- Kenya Tsuji Yuki Kitade Takuya Sumita Chihiro Tanaka
- 出版者
- The Mycological Society of Japan
- 雑誌
- Mycoscience (ISSN:13403540)
- 巻号頁・発行日
- vol.62, no.5, pp.289-296, 2021-09-20 (Released:2021-09-20)
- 参考文献数
- 29
In this study, we identified Sec5 in Bipolaris maydis, a homologue of Sec5 in Saccharomyces cerevisiae and a possible exocyst component of the fungus. To examine how Sec5 affects the life cycle of B. maydis, we generated null mutant strains of the gene (Δsec5). The Δsec5 strains showed a strong reduction in hyphal growth and a slight reduction in pathogenicity. In sexual reproduction, they possessed the ability to develop pseudothecia. However, all ascospores were aborted in any of the asci obtained from crosses between Δsec5 and the wild-type. Our cytological study revealed that the abortion was caused by impairments of the post-meiotic stages in ascospore development, where ascospore delimitation and young spore elongation occur.
2 0 0 0 OA Rapid and simple preparation of mushroom DNA directly from colonies and fruiting bodies for PCR
- 著者
- Kosuke Izumitsu Kanako Hatoh Takuya Sumita Yuki Kitade Atsushi Morita Chihiro Tanaka Abdul Gafur Akira Ohta Masataka Kawai Takashi Yamanaka Hitoshi Neda Yuko Ota
- 出版者
- The Mycological Society of Japan
- 雑誌
- Mycoscience (ISSN:13403540)
- 巻号頁・発行日
- vol.53, no.5, pp.396-401, 2012 (Released:2023-03-31)
- 被引用文献数
- 106
We have optimized a simple and rapid preparation procedure for mushroom DNA extraction from colonies on media or from fruiting bodies for PCR amplification. The protocol combines microwaving twice for 1 min, cooling for 10 min, and centrifuging for 5 min. By using this procedure, more than 100 samples of mushroom DNA can be prepared within 1 h. The DNA obtained can be used for (1) identifying mushroom species by PCR and subsequent sequencing, (2) amplifying low copy number genes (at least 2,000 bp), and (3) screening genetic transformants. This technique will contribute to the mycology of mushroom species.