著者

Hiroaki SAMEJIMA Byoung-Jae PARK

出版者

Japanese Society for Tropical Agriculture

雑誌

Tropical Agriculture and Development (ISSN:18828450)

巻号頁・発行日

vol.63, no.1, pp.12-17, 2019 (Released:2019-08-20)

参考文献数

33

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Guava leaf extract has significant application in natural remedies and cosmetics. In this study, we have focused on the total polyphenol content and inhibition activity against skin dermis enzymes (collagenase, elastase, and hyaluronidase) and carbohydrate digestion enzymes (α-amylase and α-glucosidase). Dried powdered guava leaves were extracted in 70% methanol and lyophilized to produce a crude extract. Aliquots were further divided into Hexane fr., EtOAc fr., BuOH fr., and Water fr. Total polyphenol content was the highest in the BuOH fr. among the crude extract and the four fractions. The Hexane fr. did not reach the 50% inhibitory concentration (IC50) against all the enzymes studied. The crude extract, BuOH fr., and Water fr. showed low IC50 (high inhibition activity) against collagenase, elastase, and hyaluronidase, with the BuOH fr. having the highest inhibition activity. In addition, this had high inhibition activity against α-amylase and α-glucosidase. The ability to inhibit multiple enzymes, relating to skin aging or carbohydrate digestion, suggests significant potential for using guava leaf extract in natural remedies and cosmetics.