- 著者
- Atsushi Okazawa Hiroaki Samejima Shigeru Kitani Yukihiro Sugimoto Daisaku Ohta
- 出版者
- Pesticide Science Society of Japan
- 雑誌
- Journal of Pesticide Science (ISSN:1348589X)
- 巻号頁・発行日
- pp.D21-014, (Released:2021-05-20)
- 参考文献数
- 45
- 被引用文献数
- 8
Damage caused by Orobanchaceae root parasitic weeds is a substantial agricultural problem for global food security. Many studies have been conducted to establish practical methods of control, but efforts are still required for successful management. Seed germination of root parasitic weeds requires host-derived germination stimulants including strigolactones (SLs). Studies on SLs have revealed that a butenolide ring is the essential moiety for SL activity as a germination stimulant. Interestingly, recent studies have revealed that butenolide hormones regulate the biosynthesis of secondary metabolites and mediate communication in actinomycete bacteria. Because of the structural similarity between SLs and the bacterial butenolides, we evaluated the germination stimulatory activity of butenolides isolated from Streptomyces albus J1074 on root parasitic weeds. These butenolides were found to specifically induce seed germination of Orobanche minor. Our findings contribute to understanding the molecular mechanisms of germination stimulant perception and to the development of a method for their biological control.
- 著者
- Hiroaki SAMEJIMA Byoung-Jae PARK
- 出版者
- Japanese Society for Tropical Agriculture
- 雑誌
- Tropical Agriculture and Development (ISSN:18828450)
- 巻号頁・発行日
- vol.63, no.1, pp.12-17, 2019 (Released:2019-08-20)
- 参考文献数
- 33
Guava leaf extract has significant application in natural remedies and cosmetics. In this study, we have focused on the total polyphenol content and inhibition activity against skin dermis enzymes (collagenase, elastase, and hyaluronidase) and carbohydrate digestion enzymes (α-amylase and α-glucosidase). Dried powdered guava leaves were extracted in 70% methanol and lyophilized to produce a crude extract. Aliquots were further divided into Hexane fr., EtOAc fr., BuOH fr., and Water fr. Total polyphenol content was the highest in the BuOH fr. among the crude extract and the four fractions. The Hexane fr. did not reach the 50% inhibitory concentration (IC50) against all the enzymes studied. The crude extract, BuOH fr., and Water fr. showed low IC50 (high inhibition activity) against collagenase, elastase, and hyaluronidase, with the BuOH fr. having the highest inhibition activity. In addition, this had high inhibition activity against α-amylase and α-glucosidase. The ability to inhibit multiple enzymes, relating to skin aging or carbohydrate digestion, suggests significant potential for using guava leaf extract in natural remedies and cosmetics.