- 著者
- Yasuyuki SASANO Alu KONNO Megumi NAKAMURA Akiko HENMI Miyuki MAYANAGI Mu-Chen YANG Ikuko YAO
- 出版者
- Biomedical Research Press
- 雑誌
- Biomedical Research (ISSN:03886107)
- 巻号頁・発行日
- vol.44, no.4, pp.173-179, 2023-08-03 (Released:2023-08-05)
- 参考文献数
- 12
Matrix-assisted laser desorption/ionization imaging mass spectrometry (IMS) is used to comprehensively visualize the spatial distribution of numerous biomolecules. The present study was designed to investigate the distribution of phospholipids in developing rat teeth by IMS to identify the characteristic phospholipid molecules for tooth development, and to evaluate the suitability of tissue preparation methods. Rats at postnatal day 3 were euthanized, and the resected head specimens were either fixed or not fixed with 4% paraformaldehyde (PFA), and decalcified or not decalcified in 10% ethylenediaminetetraacetic acid (EDTA) before being frozen. Subsequently, sections were prepared and mounted on glass slides coated with indium tin oxide, and analyzed by IMS. The mass spectra showed the highest peaks around m/z 706, 732, and 734 in the region of interest. Characteristic localization of signals in the tooth buds was seen around m/z 706 and 732, and a database search indicated that the corresponding molecules were phosphatidylcholines. The signals were localized to the dental papillae and enamel epithelia in the tooth buds. The PFA-fixed specimens with or without EDTA decalcification showed preserved IMS signals, while the non-fixed specimens showed fewer signals. Thus, PFA fixation with EDTA decalcification appears to be suitable for IMS analysis of calcified tissues.
- 著者
- Shuxiang LI Xin GAO Yancai WEI Gengchao ZHU Chen YANG
- 出版者
- Center for Academic Publications Japan
- 雑誌
- Journal of Nutritional Science and Vitaminology (ISSN:03014800)
- 巻号頁・発行日
- vol.62, no.6, pp.397-401, 2016 (Released:2017-02-16)
- 参考文献数
- 26
- 被引用文献数
- 22
Previous studies have identified an association between iron deficiency and thyroid function. We aimed to determine if there is a relationship between iron deficiency and thyroid function during the first trimester of pregnancy. Two thousand five hundred eighty-one pregnant women who presented for the first prenatal care were enrolled and divided into three groups, the mild iron deficiency (MID) group, iron deficiency anemia (IDA) group and normal control (NC) group, according to serum ferritin and hemoglobin levels. The former two groups can be merged into one iron deficiency (ID) group. Thyroid function parameters were compared among the three groups, including free thyroxine (FT4), thyroid stimulating hormone (TSH), total thyroxine (TT4) and thyroid peroxidase antibodies (TPOAb). Moreover, the rates of thyroid dysfunction were also compared. Our results show that pregnant women in the MID and IDA groups have higher TSH and lower FT4 status than those in the NC group (p<0.01), and the difference between the IDA group and MID group is significant (p<0.05). TPOAb in the IDA group is higher than in the MID group and NC group. Meanwhile, the rate of hypothyroidism or subclinical hypothyroidism in the IDA group was significantly higher than in the MID group and NC group (p<0.01). And the positive rate of TPOAb is also higher in the IDA group than MID group and NC group (p<0.05). Iron deficiency is related to thyroid function and could lead to hypothyroidism during early pregnancy, which could be explained by thyroid autoimmunity.
- 著者
- CHEN Zhijia LIN Chuang CHEN Yang NIVARGI Vaibhav CAO Pei
- 出版者
- The Institute of Electronics, Information and Communication Engineers
- 雑誌
- IEICE transactions on communications (ISSN:09168516)
- 巻号頁・発行日
- vol.91, no.12, pp.3842-3850, 2008-12-01
- 被引用文献数
- 1 5
With the popularity of BitTorrent-like P2P applications, improving its performance has been an active research area. Super-seeding, a special upload policy for the initial seeder, improves the efficiency in producing multiple seeds and reduces the uploading bytes of content initiators, thus being highly expected as a promising solution for improving downloading performance while decreasing uploading cost. However, the overall impacts of super seeding upon BitTorrent performance remain a question and have not been analyzed so far in literature. In this paper, we present an analytical and experimental study over the performance of super-seeding scheme. We attempt to answer the following questions: whether and how much super-seeding saves uploading cost, whether the overall downloading time is decreased by super-seeding, and in which circumstances super-seeding performs worse. Based on the seeding process, our analytical study gives formulas on the new piece distribution time, average downloading time and minimum distribution time for heterogeneous P2P file distribution system with super-seeding. Robust evidence supporting the use (or not) of super-seeding is given based on our worldwide Internet experiments over wide distribution of 250 PlanetLab nodes. With a well-designed experimental scenario, we study the overall download time and upload cost of super seeding scheme under varying seed bandwidth and peer behavior. Results show that super-seeding can save an upload ratio of 20% and does help speeding up swarms in certain modes. Tentative conclusions about the effectiveness of super-seeding and its optimal working circumstances are given with inside mechanism analyzed and negative factor identified. Our work not only provides reference for the potential adoption of super-seeding in BitTorrent and other P2P applications, but also much insights for the tussle of enhancing of Quality of Experience (QoE) and saving cost for a large-scale BitTorrent-like P2P commercial application.