- 著者
- MARC F. OXENHAM LORNA TILLEY HIROFUMI MATSUMURA LAN CUONG NGUYEN KIM THUY NGUYEN KIM DUNG NGUYEN KATE DOMETT DAMIEN HUFFER
- 出版者
- 日本人類学会
- 雑誌
- Anthropological Science (ISSN:09187960)
- 巻号頁・発行日
- vol.117, no.2, pp.107-112, 2009 (Released:2009-07-24)
- 参考文献数
- 32
- 被引用文献数
- 12 36 9
This communication documents one of the earliest verifiable cases of human paralysis associated with severe spinal pathology. A series of skeletal abnormalities is described for a young adult male (M9) from a Southeast Asian Neolithic community. Differential diagnosis suggests that M9 suffered from a severely disabling congenital fusion of the spine (Klippel–Feil Syndrome, Type III), resulting in child-onset lower body paralysis at a minimum (maximally quadriplegia). M9 experienced severe, most probably total, incapacitation for at least a decade prior to death. In the prehistoric context, this individual’s condition would have rendered him completely dependent on others for survival.
- 著者
- Kyohei MATSUI So TANABE Shuyi SUN Dung NGUYEN Takamasa KINOSHITA Yojiro YAMAMOTO Hiroshi SHIIGI
- 出版者
- The Japan Society for Analytical Chemistry
- 雑誌
- Analytical Sciences (ISSN:09106340)
- 巻号頁・発行日
- vol.36, no.12, pp.1461-1465, 2020-12-10 (Released:2020-12-10)
- 参考文献数
- 31
- 被引用文献数
- 5
Enzyme-linked immunosorbent assay (ELISA) is a widespread analytical biochemistry assay. In this work, a direct ELISA method using a metallic nanoparticle (NP)-immobilized 96-well plate was developed for high-throughput, highly sensitive fluorescence analysis. Immobilization of metallic NPs on a 96-well plate effectively amplified fluorescence signals of the assay. The silver (Ag) NP-immobilized plate showed the best fluorescence enhancement effect of all the metal-immobilized plates tested. We used the Ag NP-immobilized plate to detect biomolecules and bacteria and found that both the fluorescence intensity and the limit of detection (LOD) were strongly enhanced by more than 100 times compared with those of the unmodified 96-well plates. Quantitative and qualitative considerations for target bacteria regarding the impact of autofluorescence on detection were successfully obtained for several strains. Our results demonstrate the potential of applying Ag NPs for enhancing the efficiency of direct and indirect ELISA assays.
- 著者
- Shuyi SUN Kyohei MATSUI So TANABE Dung NGUYEN Takamasa KINOSHITA Yojiro YAMAMOTO Hiroshi SHIIGI
- 出版者
- The Japan Society for Analytical Chemistry
- 雑誌
- Analytical Sciences (ISSN:09106340)
- 巻号頁・発行日
- vol.36, no.7, pp.787-789, 2020-07-10 (Released:2020-07-10)
- 参考文献数
- 11
- 被引用文献数
- 4
In this reported work, we achieved high-throughput, highly sensitive fluorescent analysis using an enzyme-linked immunosorbent assay (ELISA) that employed a metallic nanoparticle (NP)-immobilized 96-well plate. The immobilization of metallic NPs on a 96-well plate effectively amplified fluorescent signals of the assay. The silver (Ag) NP-immobilized plate showed the best fluorescent enhancement effect of all plates immobilized by metal NPs. Our results demonstrate the potential of applying Ag NPs to enhance the efficiency of direct and indirect ELISA by the labeling of targets.