著者

Polona SMRKOLJ Vekoslava STIBILJ Ivan KREFT Emese KAPOLNA

出版者

(社)日本分析化学会

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.21, no.12, pp.1501-1504, 2005 (Released:2006-02-24)

参考文献数

30

被引用文献数

22 38

---

Pumpkins were treated by spraying the leaves in the flowering period with a water solution containing 1.5 mg Se per liter in the form of Na2SeO4. The average total selenium content of seeds was found to be 0.19 µg g-1 in nontreated pumpkins and 1.1 µg g-1 in exposed ones. For speciation analysis, enzymatic hydrolysis with different amounts of Protease XIV was carried out. Under optimal conditions of enzymatic hydrolysis, 90% of the total selenium was found in soluble forms. Separation of species was performed using HPLC on anion and cation exchange columns and for detection UV-HG-AFS was applied. In enzymatic hydrolysis extracts, the main fraction of selenium was bound as selenomethionine (SeMet), representing on average of 81 ± 8% of the total Se content in the sample.