- 著者
- Thu Thuy Bui Meng Ling Moi Kouichi Morita Futoshi Hasebe
- 出版者
- National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee
- 雑誌
- Japanese Journal of Infectious Diseases (ISSN:13446304)
- 巻号頁・発行日
- pp.JJID.2019.073, (Released:2019-10-31)
- 参考文献数
- 15
- 被引用文献数
- 7
Zika is a mosquito-borne disease that is causing significant public health threats in recent years. Zika virus (ZIKV), the causative agent of this disease, is classified into two distinct genetic lineages: Asian and African lineages. While molecular nucleic acid methods have been proved useful for the diagnosis of ZIKV infection, development of assays based on one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) offers advantages including shorter incubation times, ease of handling and rapid detection. In this study, a universal LAMP primer set was developed to target conserved sequence of known ZIKV lineages. Additionally, Af7462 and As1788 primer sets were designed based on LAMP-based SNPs typing for the specific detection of African and Asian lineages. The RT-LAMP assays detected specifically African and Asian lineages, with the limit of detection range from 0.17 FFU/ml – 2.3x102 FFU/ml. As ZIKV viremia ranges between 102 to 106 PFU/ml or 103–106 copies/mL, the data indicate that the viremia range of clinical samples is within our detection range. Because of the high specificity and sensitivity and ease of use, the results suggest the utility of the assay in early clinical diagnosis applications.
- 著者
- Duc Tuan Dinh Mai Thi Quynh Le Cuong Duc Vuong Futoshi Hasebe Kouichi Morita
- 出版者
- 日本熱帯医学会
- 雑誌
- Tropical Medicine and Health (ISSN:13488945)
- 巻号頁・発行日
- vol.39, no.1, pp.3-7, 2011 (Released:2011-03-24)
- 参考文献数
- 13
- 被引用文献数
- 22 53
We designed a new set of primers for reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) to specifically amplify the HA gene of avian influenza viruses subtype H5N1. By testing nine H5N1 virus strains and 41 clinical samples collected in Northern Vietnam, we found that the new primers showed higher sensitivity and specificity than the previously published RT-LAMP primers and were comparable to the RT-PCR method currently recommended by WHO. These results suggest that our RT-LAMP assay may be a better choice as a diagnostic tool for current H5N1 influenza virus infection.