著者
Kohei OTOMO Terumasa HIBI Takashi MURATA Hirotaka WATANABE Ryosuke KAWAKAMI Hiroshi NAKAYAMA Mitsuyasu HASEBE Tomomi NEMOTO
出版者
(社)日本分析化学会
雑誌
Analytical Sciences (ISSN:09106340)
巻号頁・発行日
vol.31, no.4, pp.307-313, 2015-04-10 (Released:2015-04-10)
参考文献数
28
被引用文献数
2 33

The temporal resolution of a two-photon excitation laser scanning microscopy (TPLSM) system is limited by the excitation laser beam’s scanning speed. To improve the temporal resolution, the TPLSM system is equipped with a spinning-disk confocal scanning unit. However, the insufficient energy of a conventional Ti:sapphire laser source restricts the field of view (FOV) for TPLSM images to a narrow region. Therefore, we introduced a high-peak-power Yb-based laser in order to enlarge the FOV. This system provided three-dimensional imaging of a sufficiently deep and wide region of fixed mouse brain slices, clear four-dimensional imaging of actin dynamics in live mammalian cells and microtubule dynamics during mitosis and cytokinesis in live plant cells.