著者

Kohei OTOMO Terumasa HIBI Takashi MURATA Hirotaka WATANABE Ryosuke KAWAKAMI Hiroshi NAKAYAMA Mitsuyasu HASEBE Tomomi NEMOTO

出版者

(社)日本分析化学会

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.31, no.4, pp.307-313, 2015-04-10 (Released:2015-04-10)

参考文献数

28

被引用文献数

2 30

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The temporal resolution of a two-photon excitation laser scanning microscopy (TPLSM) system is limited by the excitation laser beam’s scanning speed. To improve the temporal resolution, the TPLSM system is equipped with a spinning-disk confocal scanning unit. However, the insufficient energy of a conventional Ti:sapphire laser source restricts the field of view (FOV) for TPLSM images to a narrow region. Therefore, we introduced a high-peak-power Yb-based laser in order to enlarge the FOV. This system provided three-dimensional imaging of a sufficiently deep and wide region of fixed mouse brain slices, clear four-dimensional imaging of actin dynamics in live mammalian cells and microtubule dynamics during mitosis and cytokinesis in live plant cells.