著者

Kasumi Hashimoto Yusuke Kazama Hiroyuki Ichida Tomoko Abe Koji Murai

出版者

Japan Mendel Society, International Society of Cytology

雑誌

CYTOLOGIA (ISSN:00114545)

巻号頁・発行日

vol.86, no.4, pp.297-302, 2021-12-25 (Released:2021-12-24)

参考文献数

14

被引用文献数

3

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A mutant showing extra early-flowering and named extra early-flowering4 (exe4) was induced in a previous study by heavy-ion beam mutagenesis of Triticum monococcum strain KU104-1. The exe4 mutant shows heading about 45 days earlier than wild-type KU104-1 in the field. In the present study, we sought to identify the gene that was mutated in exe4 by performing a modified whole-genome sequencing analysis. This analysis exploited a short-read library preparation that uses a modified adaptor and duplex-specific nuclease (DSN) for the efficient elimination of highly repeated sequence elements within genomes. The whole-genome sequence analysis and PCR analysis using an M2 segregation line indicated that the extra-early flowering phenotype of exe4 is associated with a deletion of a gene for a WD repeat protein, named here WHEAT WD REPEAT 1 (WWDR1). Phylogenetic analysis of amino acid sequences showed that the gene is a homolog of Arabidopsis LIGHT-REGULATED WD1 (LWD1) and LWD2, which are circadian clock regulatory genes.

著者

Hiroyuki Ichida Tomohiko Kazama Shin-ichi Arimura Kinya Toriyama

出版者

Japanese Society for Plant Biotechnology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.40, no.1, pp.109-112, 2023-03-25 (Released:2023-03-25)

参考文献数

11

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A highly contiguous mitochondrial and plastid genome sequences of a japonica rice cultivar, Taichung 65, were determined by a hybrid approach with long- and short-read sequences. The assembled mitochondrial genome was 465,453 bases in length with an overall GC content of 43.8%. It was predicted to harbor 62 protein-encoding genes, 16 kinds (33 copies) of transfer RNA, and three kinds (six copies) of ribosomal RNA genes. The mitochondrial genome structure in Taichung 65 is largely the same as that of Nipponbare, but the first ∼9.5 kb sequence in Nipponbare (DQ167400) is replaced with a ∼27 kb sequence duplicated from other parts of the mitochondrial genome. Phylogenetic and sequence polymorphism analysis indicated that Taichung 65 is classified as typical japonica. The assembled plastid genome sequence was 134,551 bases in length and completely identical to the previously reported Nipponbare sequence. These near-complete organelle genome sequences will serve as fundamental resources for investigating alloplasmic cytoplasmic male sterile lines and other organelle-controlled phenomena in rice.