- 著者
- Kasumi Hashimoto Yusuke Kazama Hiroyuki Ichida Tomoko Abe Koji Murai
- 出版者
- Japan Mendel Society, International Society of Cytology
- 雑誌
- CYTOLOGIA (ISSN:00114545)
- 巻号頁・発行日
- vol.86, no.4, pp.297-302, 2021-12-25 (Released:2021-12-24)
- 参考文献数
- 14
- 被引用文献数
- 3
A mutant showing extra early-flowering and named extra early-flowering4 (exe4) was induced in a previous study by heavy-ion beam mutagenesis of Triticum monococcum strain KU104-1. The exe4 mutant shows heading about 45 days earlier than wild-type KU104-1 in the field. In the present study, we sought to identify the gene that was mutated in exe4 by performing a modified whole-genome sequencing analysis. This analysis exploited a short-read library preparation that uses a modified adaptor and duplex-specific nuclease (DSN) for the efficient elimination of highly repeated sequence elements within genomes. The whole-genome sequence analysis and PCR analysis using an M2 segregation line indicated that the extra-early flowering phenotype of exe4 is associated with a deletion of a gene for a WD repeat protein, named here WHEAT WD REPEAT 1 (WWDR1). Phylogenetic analysis of amino acid sequences showed that the gene is a homolog of Arabidopsis LIGHT-REGULATED WD1 (LWD1) and LWD2, which are circadian clock regulatory genes.
- 著者
- Ayaka Matsuta Takahiro Mayuzumi Hajime Katano Masanori Hatashita Keiichi Takagi Yoriko Hayashi Tomoko Abe Koji Murai Yusuke Kazama
- 出版者
- Japan Mendel Society, International Society of Cytology
- 雑誌
- CYTOLOGIA (ISSN:00114545)
- 巻号頁・発行日
- vol.86, no.4, pp.317-322, 2021-12-25 (Released:2021-12-24)
- 参考文献数
- 43
- 被引用文献数
- 1
A high-LET heavy-ion beam has a severe effect on survival and effectively induces chromosomal rearrangements. In this study, the effect of high-LET heavy-ion irradiation on mutation induction in the M1 generation was investigated in an inbred line of Torenia fournieri, which is a widely used horticultural plant. Dry seeds of the inbred line ‘Zairai murasaki’ were irradiated with a C-ion beam (LET: 50 keV µm−1) or Ar-ion beams (LETs: 184 keV µm−1 or 290 keV µm−1) at different doses, and then sown on 1/2 MS plates. After determining the survival rates from each irradiation condition, appropriate doses of each beam were roughly determined to produce a survival rate of 90%: 300, 75, and 50 Gy for the C-ion beam with a LET of 50 keV µm−1, Ar-ion beam with a LET of 184 keV µm−1, and Ar-ion beam with a LET of 290 keV µm−1, respectively. In the screening of branches with aberrant flowers, one and two aberrant plants were isolated from 16 and 30 M1 plants after irradiation with LETs of 184 keV µm−1 and 290 keV µm−1, respectively. However, no aberrant plants were identified in M1 plants after irradiation with a LET of 50 keV µm−1. We concluded that high-LET heavy-ion beam irradiation is effective in inducing mutations even in the M1 generation of inbred ornamental plants. This technique could be widely used for breeding ornamental plants that can be propagated vegetatively.
- 著者
- Naoki Shitsukawa Chihiro Ikari Sanae Shimada Satoshi Kitagawa Koichi Sakamoto Hiroyuki Saito Hiromichi Ryuto Nobuhisa Fukunishi Tomoko Abe Shigeo Takumi Shuhei Nasuda Koji Murai
- 出版者
- The Genetics Society of Japan
- 雑誌
- Genes & Genetic Systems (ISSN:13417568)
- 巻号頁・発行日
- vol.82, no.2, pp.167-170, 2007 (Released:2007-05-11)
- 参考文献数
- 13
- 被引用文献数
- 88 98
The einkorn wheat (Triticum monococcum) mutant, maintained vegetative phase (mvp), was induced by nitrogen ion-beam treatment and was identified by its inability to transit from the vegetative to reproductive phase. In our previous study, we showed that WAP1 (wheat APETALA1) is a key gene in the regulatory pathway that controls phase transition from vegetative to reproductive growth in common wheat. WAP1 is an ortholog of the VRN1 gene that is responsible for vernalization insensitivity in einkorn wheat. The mvp mutation resulted from deletion of the VRN1 coding and promoter regions, demonstrating that WAP1/VRN1 is an indispensable gene for phase transition in wheat. Expression analysis of flowering-related genes in mvp plants indicated that wheat GIGANTIA (GI), CONSTANS (CO) and SUPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) genes either act upstream of or in a different pathway to WAP1/VRN1.