著者

Hitoshi Oshitani The Experts Members of The National COVID-19 Cluster Taskforce at Ministry of Health Labour and Welfare Japan

出版者

National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

雑誌

Japanese Journal of Infectious Diseases (ISSN:13446304)

巻号頁・発行日

pp.JJID.2020.363, (Released:2020-06-30)

参考文献数

10

被引用文献数

57

著者

Hidekazu Nishimura Michiko Okamoto Isolde Dapat Masanori Katumi Hitoshi Oshitani

出版者

National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

雑誌

Japanese Journal of Infectious Diseases (ISSN:13446304)

巻号頁・発行日

pp.JJID.2020.902, (Released:2021-01-29)

参考文献数

8

被引用文献数

25

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Green tea extracts effectively inactivated SARS-CoV-2 in vitro in a dose-dependent manner. Serially 10-fold diluted solutions of catechin mixture reagent from green tea were mixed with the viral culture fluid at a volume ratio of nine to one, respectively, and kept at room temperature for 5 min. The solution of 10 mg/mL catechin reagent reduced the viral titer by 4.2 log and 1.0 mg/mL solution reduced only by one log. Pre-infection treatment of the cells with the reagent alone did not affect the viral growth. In addition, cells treated with only the reagent was assayed for host-cell viability using the WST-8 system and almost no host-cell damage by the treatment was observed. These findings suggested that the direct treatment of virus with the reagent before inoculation decreased the viral activity and that catechins might have a potential to suppress the SARS-CoV-2 infection.

著者

Hidekazu Nishimura Michiko Okamoto Isolde Dapat Masanori Katsumi Hitoshi Oshitani

出版者

National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

雑誌

Japanese Journal of Infectious Diseases (ISSN:13446304)

巻号頁・発行日

vol.74, no.5, pp.421-423, 2021-09-30 (Released:2021-09-22)

参考文献数

8

被引用文献数

1 25

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Green tea extracts effectively inactivated severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in vitro in a dose-dependent manner. Ten-fold serially diluted solutions of catechin mixture reagent from green tea were mixed with the viral culture fluid at a volume ratio of 9:1, respectively, and incubated at room temperature for 5 min. The solution of 10 mg/mL catechin reagent reduced the viral titer by 4.2 log and 1.0 mg/mL solution by one log. Pre-infection treatment of cells with the reagent alone did not affect viral growth. In addition, cells treated with only the reagent were assayed for host cell viability using the WST-8 system, and almost no host cell damage by the treatment was observed. These findings suggested that the direct treatment of virus with the reagent before inoculation decreased the viral activity and that catechins might have the potential to suppress SARSCoV-2 infection.

著者

Hitoshi Oshitani The Expert Members of The National COVID-19 Cluster Taskforce at The Ministry of Health Labour and Welfare Japan

出版者

National Institute of Infectious Diseases

雑誌

Japanese Journal of Infectious Diseases (ISSN:13446304)

巻号頁・発行日

vol.73, no.6, pp.491-493, 2020-11-30 (Released:2020-11-24)

参考文献数

10

被引用文献数

29 57