- 著者
- Xiaochen Liu Wenxiao Jia Hong Wang Yunling Wang Jingxun Ma Hao Wang Xuan Zhou Guohua Li
- 出版者
- National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee
- 雑誌
- Japanese Journal of Infectious Diseases (ISSN:13446304)
- 巻号頁・発行日
- vol.68, no.2, pp.89-97, 2015 (Released:2015-03-23)
- 参考文献数
- 34
- 被引用文献数
- 4 8
This study aimed to establish a spinal tuberculosis model by implanting Mycobacterium tuberculosis strain H37Rv into the lumbar vertebral body of New Zealand white rabbits. A hole was first drilled into the top of the 6th lumbar vertebra of each rabbit, which was then filled with a gelatin sponge to adsorb 0.2 ml of M. tuberculosis suspension (107 CFU /ml) for the infection group or normal saline for the control group. The holes were then closed with sutures. CT findings demonstrated that 5 and 10 rabbits developed spinal tuberculosis at 4 and 8 weeks, respectively, after this procedure. MRI examinations revealed that 7 and 15 rabbits had positive results at 4 and 8 weeks, respectively, after this procedure. HE staining of the vertebral body and paravertebral soft tissue biopsies of infected rabbits indicated inflammatory cell infiltration or necrosis in 15 rabbits. M. tuberculosis was cultured in 67% of the abscesses. The modeling success rate was 68.1%. By implanting an appropriate dosage of M. tuberculosis strain H37Rv into a local lumbar vertebral body of New Zealand white rabbits, we successfully established a spinal tuberculosis model, the pathological changes of which are similar to those of human spinal tuberculosis.
- 著者
- Yuan Tao Pengfei Jiang Yuhua Wei Ping Wang Xiaoling Sun Hong Wang
- 出版者
- 東北ジャーナル刊行会
- 雑誌
- The Tohoku Journal of Experimental Medicine (ISSN:00408727)
- 巻号頁・発行日
- vol.240, no.3, pp.209-214, 2016 (Released:2016-11-11)
- 参考文献数
- 44
- 被引用文献数
- 11
Dry form of age-related macular degeneration (AMD) constitutes 90% of AMD cases, and it is characterized by the formation of drusen under the retina and the slow breakdown of the light-sensing cells in the macula, which causes a gradual loss of central vision. Since oxidative stress is involved in the pathogenesis of dry AMD, α-lipoic acid (LA) with antioxidant properties was selected, and its effect on anti-oxidative markers and visual quality in patients with dry AMD was assessed. A total of 100 dry AMD patients (60-83 years old) were randomly assigned to LA treatment group (n = 50) and placebo control group (n = 50). We measured the serum superoxide dismutase (SOD) activity, an important marker of antioxidant defense, best-corrected visual acuity (BCVA), contrast sensitivity, and Chinese-Version Low Vision Quality of Life (CLVQOL) before and after LA or placebo intervention. Pearson correlation coefficients were calculated to explore the relationship between contrast sensitivity values and CLVQOL scores. There was a statistically significant increase in serum SOD activity after LA intervention. The CLVQOL score was improved significantly after LA treatment. The contrast sensitivity measured at middle and low spatial frequency was significantly higher after LA treatment. CLVQOL scores were positively correlated with contrast sensitivity at low spatial frequency (3 cyc/degree) in LA-treated group. These results indicate that LA treatment improves vision-related quality of life in patients with dry AMD probably by increasing antioxidant activity. Thus, LA can be regarded as a promising agent for the treatment of AMD.