- 著者
-
永井 教之
村田 勝
辻極 秀次
井上 正久
長塚 仁
中野 敬介
赤木 巧
衣田 圭宏
完山 学
秦 春林
張 紹全
此内 浩信
韓 松
黄 炳珍
譚 軍
劉 桂需
Gomah Atia
日比 一光
Noriyuki Nagai
Masaru Murata
Hidetsugu Tsujigiwa
Masahisa Inoue
Hitoshi Nagatsuka
Keisuke Nakano
Takumi Akagi
Yoshihiro Kinuta
Manabu Kanyama
Chun-Lin Qin
Shao-Quan Zhang
Hironobu Konouchi
Song Han
Bing-Zhen Huang
Jun Tan
Gui-Ru Liu
Atia Gomah
kazumitsu Hibi
岡山大学歯学部口腔病理学講座
岡山大学歯学部口腔病理学講座
岡山大学歯学部口腔病理学講座
岡山大学歯学部口腔病理学講座
岡山大学歯学部口腔病理学講座
岡山大学歯学部口腔病理学講座
岡山大学歯学部口腔病理学講座
岡山大学歯学部口腔病理学講座
岡山大学歯学部口腔病理学講座
岡山大学歯学部口腔病理学講座
岡山大学歯学部口腔病理学講座
岡山大学歯学部口腔病理学講座
岡山大学歯学部口腔病理学講座
岡山大学歯学部口腔病理学講座
岡山大学歯学部口腔病理学講座
岡山大学歯学部口腔病理学講座
岡山大学歯学部口腔病理学講座
岡山大学歯学部口腔病理学講座
Department of Oral Pathology Okayama University Dental School
Department of Oral Pathology Okayama University Dental School
Department of Oral Pathology Okayama University Dental School
Department of Oral Pathology Okayama University Dental School
Department of Oral Pathology Okayama University Dental School
Department of Oral Pathology Okayama University Dental School
Department of Oral Pathology Okayama University Dental School
Department of Oral Pathology Okayama University Dental School
Department of Oral Pathology Okayama University Dental School
Department of Oral Pathology Okayama University Dental School
Department of Oral Pathology Okayama University Dental School
Department of Oral Pathology Okayama University Dental School
Department of Oral Pathology Okayama University Dental School
Department of Oral Pathology Okayama University Dental School
Department of Oral Pathology Okayama University Dental School
Department of Oral Pathology Okayama University Dental School
Department of Oral Pathology Okayama University Dental School
Department of Oral Pathology Okayama University Dental School
- 雑誌
- 日本硬組織研究技術学会雑誌 = Journal of hard tissue biology (ISSN:13405179)
- 巻号頁・発行日
- vol.5, no.2, pp.163-173, 1996-07-10
- 参考文献数
- 32
- 被引用文献数
-
3
A modofied method was established to extracted BMP from bovine bone. SDS-PAGE and Westen blotting were apllied to analize the components of this partially purified BMP. In addition the amino acid sequence was studied using protein sequencer to analyze the unknown bands. Type I collagen derived from bone was used as a carrier whose properties and advantages were studied. The following conclusions are made from this study. 1. The limitation of molecluar weight in tbe early step of BMP purification procedure improves purification efficiency and stability, which makes the applicadon of BMP in big animalos possible. 2. In this method the purified BMP product also contains histone H3, histone 2B and other unknown proteins as well as BMP-2. 3. The atelocollagen derived from bone has proved to be an useful carrier for BMP. This carrier shows characteristics of self absorption and recalcification when embedded in tissues. 4. The minimun bone induction dose of BMP at each step (G-Ext:500μg, Hep:30μg, S200:50μg) and the optimal bone induction dose(G-Ext:5.0mg, Hep:2.0mg, S200:0.3mg) are determined, which makes the applicalion of BMP in cellular biology possible.