- 著者
- Kanjana Worarad Xiaonan Xie Inna Martha Rumainum Chairat Burana Kenji Yamane
- 出版者
- 一般社団法人 園芸学会
- 雑誌
- The Horticulture Journal (ISSN:21890102)
- 巻号頁・発行日
- pp.OKD-043, (Released:2016-12-29)
- 被引用文献数
- 5
Fluridone, an inhibitor of carotenoid biosynthesis, is known to prevent abscisic acid (ABA) biosynthesis and to affect the germination and dormancy of seeds in several plant species. This study investigated the effects of fluridone on seed germination of the ornamental peach ‘Yaguchi’, and on the transcript levels of genes related to seed dormancy in this plant. Seeds were rinsed for 2 days under running tap water (RS), then soaked for 1 day in 100 μM fluridone (F), and kept at 5°C for 2 weeks (2W). The germination rate significantly increased from 0% to 9.5% in the RS+F treatment and to 71.4% in the RS+F+2W treatment, while no germination occurred in the RS and RS+2W treatments. Seedlings in RS+F and RS+F+2W treatments formed dwarf shoots, i.e. about 10 cm, and morphological lesions on the leaves. The ABA content in embryonic axes decreased after RS and increased with RS+2W. It was decreased by the RS+F+2W treatment. RS+F+2W downregulated ABA-hy3, which encodes enzymes with key roles in ABA catabolism, while its effects on 9-cis-epoxycarotenoid dioxygenase (NCED1) in relation to ABA synthesis fluctuated. In contrast, Empfindlicherim Dunkelroten Licht 1 (EID1) was upregulated after fluridone treatment, suggesting that fluridone may activate positive ABA signaling pathways. Expression of GA2-oxidase8 (GA2-ox8) was not affected by fluridone. MADS-box protein JOINTLESS (LeMADS) and Late embryogenesis abundant protein D-34 (LEA D-34) were downregulated in the RS+F+2W treatment, suggesting that the expression of these genes are controlled by low temperature and the ABA inhibitor, and are involved in seed dormancy. These results suggest that ABA inhibitor treatments can be an alternative method to promote germination by controlling ABA content and its metabolism, and consequently changing expression of certain ABA- and dormancy-related genes including ABA-hy3, EID1, LeMADS, and LEA D-34, even under insufficient chilling conditions.
- 著者
- Inna Martha Rumainum Kanjana Worarad Yoshikazu Yamaki Kenji Yamane
- 出版者
- 一般社団法人 園芸学会
- 雑誌
- The Horticulture Journal (ISSN:21890102)
- 巻号頁・発行日
- pp.MI-077, (Released:2015-11-21)
- 被引用文献数
- 6
A red-fleshed peach (Prunus persica L. Batsch ‘Tenshin Suimitsuto’; ‘Tianjinshuimi’ in Chinese) was used to study the mechanisms of skin and flesh pigmentation. Anthocyanin accumulation was detected at the pre-endocarp-hardening and maturation stages in skin, while it appeared from maturation stage in flesh. The flesh at harvest stage contained approximately 1.1 mg·g−1 FW of anthocyanin. In contrast, flavonoid and phenolic compounds were found in all stages. The final phenolics concentration was 1.6 mg·g−1 FW. To study the effects of light, fruits were on-tree bagged in double-layered paper bags. The total anthocyanin content in the skin was significantly lowered by bagging, but not in the flesh. This suggested that flesh tissue pigmentation was less sensitive to light intensity than that of skin tissue, and thus, anthocyanin accumulation in flesh occurred even under low light conditions. Dark treatments, such as triple-layered paper bagging, significantly reduced anthocyanin and phenolic concentrations in the skin and flesh. A combined treatment of darkness and an auxin polar transport inhibitor, 2,3,5-triiodobenzoic acid (TIBA), had no effect on anthocyanin concentration in skin and flesh. However, total flavonoids in the skin was increased by TIBA treatments under darkness. Factors affecting anthocyanin accumulation in the flesh tissue are discussed.