著者

Kanji MIYABE Ryohei TAKAHASHI Youki SHIMAZAKI

出版者

(社)日本分析化学会

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.31, no.10, pp.1019-1025, 2015-10-10 (Released:2015-10-10)

参考文献数

40

被引用文献数

3 18

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We developed moment analysis of affinity kinetics by chromatographic capillary electrophoresis (MKCCE) method for the kinetic study of intermolecular interactions. Association and dissociation rate constants of the interaction in a micellar electrokinetic chromatography (MEKC) system between thymol and sodium dodecylsulfate micelle were determined by the MKCCE method. It is a method based on the moment theory for the kinetic study of intermolecular interactions under the conditions that neither immobilization nor chemical modification of molecules is required. In CCE mode, experimental conditions are controlled so that the migration of solute-micelle complex is stopped and only solute molecules migrate in a capillary. Mass transfer behavior of solute molecules in the CCE system is analogous to that in a chromatographic system. However, because it was difficult in practice to really perform CE experiments under the CCE conditions, CE data were measured with changing experimental conditions, i.e., applied pressure, under the conditions that the migration velocity of solute-micelle complex was around zero. The rate constants could be analytically determined from the CE data. In the MKCCE method, it is not necessary to fit elution curves numerically calculated to those experimentally measured for the determination of the rate constants. Regarding the interaction between thymol and SDS micelles, association equilibrium constant and association and dissociation rate constants were determined as 6.35 × 103 dm3 mol−1, 5.6 × 104 dm3 mol−1 s−1, and 8.7 s−1, respectively. It was demonstrated that the MKCCE method was effective for the kinetic study of intermolecular interactions.

著者

Kanji Miyabe Youki Shimazaki

出版者

(社)日本化学会

雑誌

Bulletin of the Chemical Society of Japan (ISSN:00092673)

巻号頁・発行日

pp.20150203, (Released:2015-08-25)

被引用文献数

12

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We developed a capillary electrophoretic (CE) method for the kinetic study of intermolecular interactions. Neither immobilization nor chemical modification, e.g., fluorescence labelling, of solute and ligand molecules is required. A concept of chromatographic capillary electrophoresis (CCE) was introduced as an experimental CE procedure. In the CCE mode, the migration of solute-ligand complex in a capillary is stopped. Only solute molecule migrates and is detected. New moment equations were developed, which were essential for determining association and dissociation rate constants from elution peak profiles measured under the CCE conditions. The combination of the CCE concept and the moment theory leads to the CE method, i.e., moment analysis by CCE (MACCE). However, because it was hard to really perform CE experiments under the CCE conditions, we also developed practical experimental and data analysis procedures for the MACCE measurement. The reaction rate constants were analytically determined by the MACCE method for the formation and dissociation of the inclusion complex between thymol and sulfated-β-cyclodextrin. It is unnecessary to fit elution curves numerically calculated to those experimentally measured for determining the rate constants. It was demonstrated that the MACCE method was effective for the kinetic study of intermolecular interactions.