著者

小泉 稔 堀口 幸太郎 冨田 陽子 KATO Yukio HARIGAYA Toshio

出版者

日本繁殖生物学会

雑誌

家畜繁殖研究會誌 (ISSN:09168818)

巻号頁・発行日

vol.49, no.6, pp.465-472, 2003

被引用文献数

1 7

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In this study, we attempted to examine the presence of prolactin (PRL) messenger ribonucleic acid (mRNA) and protein in the mouse nipple and mammary gland in pregnancy and lactation. PRL-like substances were found by immunohistochemistry using an antibody against the mouse PRL (mPRL) in the sebaceous gland cells of the nipple during late pregnancy and lactation, and the cistern of alveoli in mammary glands during lactation. Western blot analysis of proteins extracted from the nipple and the mammary gland showed immunoreactive bands corresponding to molecular weights of approximate 16 kDa and 32 kDa, respectively. The expression of mRNA for mPRL in the nipple and mammary gland during late pregnancy and lactation was demonstrated by reverse transcription-polymerase chain reaction (RT-PCR), Southern blotting, and nucleotide sequence analyses. These results suggest that mPRL mRNA and its translation product are synthesized in the mouse nipple.

著者

Tsutsumi Shinichi Shimazu Atsushi Miyazaki Kazuko Pan Haiou Koike Chika Yoshida Eri Takagishi Kenji Kato Yukio

出版者

Elsevier

雑誌

Biochemical and Biophysical Research Communications (ISSN:0006291X)

巻号頁・発行日

vol.288, no.2, pp.413-419, 2001-10-26

被引用文献数

11 473

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Mesenchymal stem cells (MSC) that can differentiate to various connective tissue cells may be useful for autologous cell transplantation to defects of bone, cartilage, and tendon, if MSC can be expanded in vitro. However, a short life span of MSC and a reduction in their differentiation potential in culture have limited their clinical application. The purpose of this study is to identify a growth factor(s) involved in self-renewal of MSC and the maintenance of their multilineage differentiation potential. Fibroblast growth factor-2 (FGF-2) markedly increased the growth rate and the life span of rabbit, canine, and human bone marrow MSC in monolayer cultures. This effect of FGF-2 was more prominent in low-density cultures than in high-density cultures. In addition, all MSC expanded in vitro with FGF-2, but not without FGF-2, differentiated to chondrocytes in pellet cultures. The FGF(+) MSC also retained the osteogenic and adipogenic potential throughout many mitotic divisions. These findings suggest that FGFs play a crucial role in self-renewal of MSC.

著者

Liu Ke-Xin Kato Yukio Kaku Tai-ichi SUGIYAMA Yuichi

出版者

公益社団法人日本薬学会

雑誌

Biological & pharmaceutical bulletin (ISSN:09186158)

巻号頁・発行日

vol.21, no.1, pp.44-49, 1998-01-15

被引用文献数

3 51

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The effect of human placental extract (HPE) on liver regenaration in rats was investigated. After intravenous administration of HPE to α-naphthylisothiocyanate (ANIT)-intoxicated rats, the labeling index in hepatocytes was significantly increased to a level 16.5 times higher than that of the control. A 1/500 dilution of HPE directly stimulated DNA synthesis of the hepatocytes in primary culture. HPE heated at 121℃ did not stimulate the labeling index in vivo or hepatocyte DNA synthesis in primary culture, suggesting that HPE contains heatunstable but potent mitogens for hepatocytes. HPE contains hepatocyte growth factor (HGF), but the mitogenic effect of HPE cannot be explained by the effect exerted by HGF alone, since both the labeling index in vivo and hepatocellular DNA synthesis in vitro stimulated by HPE were much higher than those stimulated by HGF alone when the applied doses of HGF were set to be almost the same level between each case. When HPE was fractionated on a heparin-sepharose column, the mitogenic effect of HPE was found to be located mainly in the heparin-bound fraction. Hepatocyte DNA synthesis induced by this fraction was enhanced cooperatively by the heparin-unbound fraction, suggesting that there are some modulators in the heparin-unbound fraction which enhance the proliferative activity of the heparin-bound fraction by a synergetic mechanism. Both HPE and heated HPE completely recovered the biochemical marker activity for liver function (glutamic-pyruvic transaminase, GPT;alkaline phosphatase, ALP; lactate dehydrogenase, LAP; γ-glutamyltransferase, γ-GTP activities and the bilirubin concentration) almost to the control level in the serum of ANIT-intoxicated rats, indicating that HPE also contains a heat-stable fraction which repairs liver function.