著者

Takeharu KANEDA Tomoe FUJIEDA Yuta ETO Yuta NAGAI Noriyasu SASAKI Tsuyoshi TAJIMA Norimoto URAKAWA Kazumasa SHIMIZU

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0020, (Released:2012-05-29)

被引用文献数

2 5

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To elucidate the role of glycogen in the contraction on tracheal smooth muscle, we investigated the changes in the glycogen contents of bovine trachea contraction induced by high K+ and hypoxia (achieved by bubbling N2 instead of O2), either in a glucose-free condition or in the presence of iodoacetic acid (IAA), an inhibitor of glycolysis. The hyperosmotic addition of 65 mM KCl (H-65 K+) induced a sustained contraction. A glucose-free condition did not affect H-65 K+-induced contraction. However, hypoxia slightly inhibited the contraction, and glucose-free PSS with hypoxia or IAA remarkably inhibited the H-65 K+-induced contraction. H-65 K+ induced a sustained increase in reduced pyridine nucleotide (PNred) fluorescence, representing glycolysis activity. Hypoxia alone slightly enhanced PNred fluorescence and when combined with a glucose-free condition, remarkably enhanced the H-65 K+-induced PNred fluorescence. IAA inhibited PNred fluorescence. In the presence of H-65 K+, a glucose-free condition, hypoxia and the combination of glucose-free PSS and hypoxia decreased the glycogen contents. However, IAA had no effect on glycogen contents. Although hypoxia or glucose-free PSS did not affect PCr and ATP contents, the combination of hypoxia and glucose-free PSS or IAA induced a gradual decrease of PCr content. In conclusion, we suggest that the endogenous glycogen was utilized to increase the activity of glycolysis for maintaining the high K+-induced contraction on the bovine trachea in glucose-free and/or hypoxic condition.