- 著者
- Noriyasu SASAKI Miwa ISHIBASHI Satoshi SOETA
- 出版者
- 公益社団法人 日本獣医学会
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- pp.13-0131, (Released:2013-05-28)
Retinol-binding protein 4 (RBP4) is a specific transporter of retinol and was recently identified as an adipokine potentially involved in type 2 diabetes in humans and rodents. However, the function and structure of feline RBP4 have not been reported. In this study, we describe the molecular cloning and expression analysis of feline RBP4. The complete feline RBP4 cDNA encodes a precursor protein comprising an 18 amino acid signal peptide and a 183 amino acid mature protein. Feline RBP4 was mapped to chromosome D2. Mature feline RBP4 is 83–94% homologous to the RBPs of humans, cows and rodents. RT-PCR analysis revealed feline RBP4 expression in liver and adipose tissues.
- 著者
- Takeharu KANEDA Tomoe FUJIEDA Yuta ETO Yuta NAGAI Noriyasu SASAKI Tsuyoshi TAJIMA Norimoto URAKAWA Kazumasa SHIMIZU
- 出版者
- 公益社団法人 日本獣医学会
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- pp.12-0020, (Released:2012-05-29)
- 被引用文献数
- 2 5
To elucidate the role of glycogen in the contraction on tracheal smooth muscle, we investigated the changes in the glycogen contents of bovine trachea contraction induced by high K+ and hypoxia (achieved by bubbling N2 instead of O2), either in a glucose-free condition or in the presence of iodoacetic acid (IAA), an inhibitor of glycolysis. The hyperosmotic addition of 65 mM KCl (H-65 K+) induced a sustained contraction. A glucose-free condition did not affect H-65 K+-induced contraction. However, hypoxia slightly inhibited the contraction, and glucose-free PSS with hypoxia or IAA remarkably inhibited the H-65 K+-induced contraction. H-65 K+ induced a sustained increase in reduced pyridine nucleotide (PNred) fluorescence, representing glycolysis activity. Hypoxia alone slightly enhanced PNred fluorescence and when combined with a glucose-free condition, remarkably enhanced the H-65 K+-induced PNred fluorescence. IAA inhibited PNred fluorescence. In the presence of H-65 K+, a glucose-free condition, hypoxia and the combination of glucose-free PSS and hypoxia decreased the glycogen contents. However, IAA had no effect on glycogen contents. Although hypoxia or glucose-free PSS did not affect PCr and ATP contents, the combination of hypoxia and glucose-free PSS or IAA induced a gradual decrease of PCr content. In conclusion, we suggest that the endogenous glycogen was utilized to increase the activity of glycolysis for maintaining the high K+-induced contraction on the bovine trachea in glucose-free and/or hypoxic condition.