- 著者
- Masaki Michishita Tatsuya Uto Ryota Nakazawa Hisashi Yoshimura Kikumi Ogihara Yuko Naya Tsuyoshi Tajima Daigo Azakami Seigo Kishikawa Toshiro Arai Kimimasa Takahashi
- 出版者
- The Japanese Pharmacological Society
- 雑誌
- Journal of Pharmacological Sciences (ISSN:13478613)
- 巻号頁・発行日
- vol.121, no.4, pp.339-342, 2013-04-20 (Released:2013-04-19)
- 参考文献数
- 15
- 被引用文献数
- 8 15
Canine hemangiopericytoma (CHP) is characterized by frequent local recurrence and increased invasiveness. Vascular endothelial growth factor (VEGF) is a key regulator of angiogenesis in tumors. The aim of the present study was to investigate the effect of a single dose of bevacizumab on a xenograft model of CHP. VEGF protein was secreted from cultured CHP cells and interacted with bevacizumab. Bevacizumab treatment suppressed tumor growth by inhibiting tumor angiogenesis, whereas no significant differences were observed in the proliferation index and apoptosis rates of treated and untreated mice. Thus, bevacizumab had antitumor effects in a xenograft model of CHP.
- 著者
- Masaki MICHISHITA Aya OHTSUKA Rei NAKAHIRA Tsuyoshi TAJIMA Takayuki NAKAGAWA Nobuo SASAKI Toshiro ARAI Kimimasa TAKAHASHI
- 出版者
- 公益社団法人 日本獣医学会
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- pp.15-0550, (Released:2015-11-28)
- 被引用文献数
- 1 22
Feline mammary carcinomas are characterized by rapid progression and metastases. Vascular endothelial growth factor (VEGF) is a key regulator of tumor angiogenesis, proliferation and metastasis. The present study aimed to investigate the effects of a single drug therapy of bevacizumab on a xenograft model of feline mammary carcinoma expressing VEGF protein. Bevacizumab treatment suppressed tumor growth by inhibiting angiogenesis and enhancing apoptosis; however, it did not affect the tumor proliferation index. Thus, bevacizumab had anti-tumor effects on a xenograft model, and this may be useful for the treatment of feline mammary carcinoma.
- 著者
- Takeharu KANEDA Tomoe FUJIEDA Yuta ETO Yuta NAGAI Noriyasu SASAKI Tsuyoshi TAJIMA Norimoto URAKAWA Kazumasa SHIMIZU
- 出版者
- 公益社団法人 日本獣医学会
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- pp.12-0020, (Released:2012-05-29)
- 被引用文献数
- 2 5
To elucidate the role of glycogen in the contraction on tracheal smooth muscle, we investigated the changes in the glycogen contents of bovine trachea contraction induced by high K+ and hypoxia (achieved by bubbling N2 instead of O2), either in a glucose-free condition or in the presence of iodoacetic acid (IAA), an inhibitor of glycolysis. The hyperosmotic addition of 65 mM KCl (H-65 K+) induced a sustained contraction. A glucose-free condition did not affect H-65 K+-induced contraction. However, hypoxia slightly inhibited the contraction, and glucose-free PSS with hypoxia or IAA remarkably inhibited the H-65 K+-induced contraction. H-65 K+ induced a sustained increase in reduced pyridine nucleotide (PNred) fluorescence, representing glycolysis activity. Hypoxia alone slightly enhanced PNred fluorescence and when combined with a glucose-free condition, remarkably enhanced the H-65 K+-induced PNred fluorescence. IAA inhibited PNred fluorescence. In the presence of H-65 K+, a glucose-free condition, hypoxia and the combination of glucose-free PSS and hypoxia decreased the glycogen contents. However, IAA had no effect on glycogen contents. Although hypoxia or glucose-free PSS did not affect PCr and ATP contents, the combination of hypoxia and glucose-free PSS or IAA induced a gradual decrease of PCr content. In conclusion, we suggest that the endogenous glycogen was utilized to increase the activity of glycolysis for maintaining the high K+-induced contraction on the bovine trachea in glucose-free and/or hypoxic condition.