- 著者
-
Takashi Okubo
Takahiro Tsukui
Hiroko Maita
Shinobu Okamoto
Kenshiro Oshima
Takatomo Fujisawa
Akihiro Saito
Hiroyuki Futamata
Reiko Hattori
Yumi Shimomura
Shin Haruta
Sho Morimoto
Yong Wang
Yoriko Sakai
Masahira Hattori
Shin-ichi Aizawa
Kenji V. P. Nagashima
Sachiko Masuda
Tsutomu Hattori
Akifumi Yamashita
Zhihua Bao
Masahito Hayatsu
Hiromi Kajiya-Kanegae
Ikuo Yoshinaga
Kazunori Sakamoto
Koki Toyota
Mitsuteru Nakao
Mitsuyo Kohara
Mizue Anda
Rieko Niwa
Park Jung-Hwan
Reiko Sameshima-Saito
Shin-ichi Tokuda
Sumiko Yamamoto
Syuji Yamamoto
Tadashi Yokoyama
Tomoko Akutsu
Yasukazu Nakamura
Yuka Nakahira-Yanaka
Yuko Takada Hoshino
Hideki Hirakawa
Hisayuki Mitsui
Kimihiro Terasawa
Manabu Itakura
Shusei Sato
Wakako Ikeda-Ohtsubo
Natsuko Sakakura
Eli Kaminuma
Kiwamu Minamisawa
- 出版者
- Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles
- 雑誌
- Microbes and Environments (ISSN:13426311)
- 巻号頁・発行日
- pp.1203230372, (Released:2012-03-28)
- 参考文献数
- 1
- 被引用文献数
-
37
53
Bradyrhizobium sp. S23321 is an oligotrophic bacterium isolated from paddy field soil. Although S23321 is phylogenetically close to Bradyrhizobium japonicum USDA110, a legume symbiont, it is unable to induce root nodules in siratro, a legume often used for testing Nod factor-dependent nodulation. The genome of S23321 is a single circular chromosome, 7,231,841 bp in length, with an average GC content of 64.3%. The genome contains 6,898 potential protein-encoding genes, one set of rRNA genes, and 45 tRNA genes. Comparison of the genome structure between S23321 and USDA110 showed strong colinearity; however, the symbiosis islands present in USDA110 were absent in S23321, whose genome lacked a chaperonin gene cluster (groELS3) for symbiosis regulation found in USDA110. A comparison of sequences around the tRNA-Val gene strongly suggested that S23321 contains an ancestral-type genome that precedes the acquisition of a symbiosis island by horizontal gene transfer. Although S23321 contains a nif (nitrogen fixation) gene cluster, the organization, homology, and phylogeny of the genes in this cluster were more similar to those of photosynthetic bradyrhizobia ORS278 and BTAi1 than to those on the symbiosis island of USDA110. In addition, we found genes encoding a complete photosynthetic system, many ABC transporters for amino acids and oligopeptides, two types (polar and lateral) of flagella, multiple respiratory chains, and a system for lignin monomer catabolism in the S23321 genome. These features suggest that S23321 is able to adapt to a wide range of environments, probably including low-nutrient conditions, with multiple survival strategies in soil and rhizosphere.