著者

筒井 正明 奥野 充 藤沢 康弘 小林 哲夫 Tsutsui Masaaki Okuno Mitsuru Fujisawa Yasuhiro Kobayashi Tetsuo

出版者

福岡大学研究推進部

雑誌

福岡大学理学集報 = Fukuoka University Science Reports (ISSN:0386118X)

巻号頁・発行日

vol.49, no.2, pp.89-97, 2019-09

著者

Makita Tomohiro Katsuyama Yoko Tani Shuji SUZUKI Hayato KATO Naoki TODD Richard B. HYNES Michael J. TSUKAGOSHI Norihiro KATO Masashi KOBAYASHI Tetsuo

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Bioscience, biotechnology, and biochemistry (ISSN:09168451)

巻号頁・発行日

vol.73, no.2, pp.391-399, 2009-02-23

被引用文献数

3 35

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AmyR is a Zn(II)<SUB>2</SUB>Cys<SUB>6</SUB> transcriptional activator that regulates expression of the amylolytic genes in <I>Aspergillus</I> species. Subcellular localization studies of GFP-fused AmyR in <I>A. nidulans</I> revealed that the fusion protein preferentially localized to the nucleus in response to isomaltose, the physiological inducer of the amylolytic genes. The C-terminal domains of AmyR, designated MH3 (residues 419–496) and MH4 (residues 516–542), were essential for sensing the inducing stimulus and regulating the subcellular localization. The MH2 domain (residues 234–375) located in the middle of AmyR was required for transcriptional activation of the target genes, and the nuclear localization signals were identified within the N-terminal Zn(II)<SUB>2</SUB>Cys<SUB>6</SUB> DNA binding motif.