- 著者
- Tadashi KIHO Shigeyuki USUI Kazuyuki HIRANO Koichi AIZAWA Takahiro INAKUMA
- 出版者
- Japan Society for Bioscience, Biotechnology, and Agrochemistry
- 雑誌
- Bioscience, Biotechnology, and Biochemistry (ISSN:09168451)
- 巻号頁・発行日
- vol.68, no.1, pp.200-205, 2004 (Released:2004-01-24)
- 参考文献数
- 31
- 被引用文献数
- 67
A water-soluble and low-molecular-weight fraction (SB) was obtained from tomato paste. The effects of SB on the formation of advanced glycation end-products (AGE) in protein glycation were studied by the methods of specific fluorescence, ELISA and a Western blot analysis, using the anti-AGE antibody after incubating protein with sugar. The results suggest that SB had strong inhibitory activity, in comparison with aminoguanidine as a positive control, and that the inhibitory mechanism of SB differed from that of aminoguanidine to involve trapping of reactive dicarbonyl intermediates in the early stage of glycation. SB contained an antioxidant, rutin, which showed potent inhibitory activity. The results also suggest that rutin chiefly contributed to inhibiting the formation of AGE, and that other compounds in SB may also have been related to the activity.
- 著者
- Koichi AIZAWA Takahiro INAKUMA
- 出版者
- Japanese Society for Food Science and Technology
- 雑誌
- Food Science and Technology Research (ISSN:13446606)
- 巻号頁・発行日
- vol.13, no.3, pp.247-252, 2007-08-25 (Released:2007-10-04)
- 参考文献数
- 21
- 被引用文献数
- 40 61
The carotenoid content, potentially an antioxidant, of 70 vegetables in Japan was determined by reversed-phase HPLC. α-Carotene was detected in 55 of 70 vegetable samples; β-carotene, in all 70; lycopene, in four types of tomatoes and Kintoki carrot; capsanthin, in only two types of sweet peppers; lutein, in 68 of the 70; zeaxanthin, in 30 of the 70; and β-cryptoxanthin in 12 of the 70 samples. All leafy vegetables contained relatively high levels of β-carotene and lutein. In contrast, fruits showed variable carotenoid compositions. A high content of lycopene was quantified in tomatoes and capsanthin was found only in red sweet peppers. In root crops, orange carrots contained high levels of α- and β-carotene, and a high level of lycopene was found in the Kintoki carrot. In other crops, such as head, beans, pods, inflorescences, and spears, carotenoid content was not found to be high in this study.