著者

Mamoru Oshiki Lin Gao Lei Zhang Satoshi Okabe

出版者

Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

雑誌

Microbes and Environments (ISSN:13426311)

巻号頁・発行日

vol.37, no.2, pp.ME21092, 2022 (Released:2022-04-12)

参考文献数

47

被引用文献数

1

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Anammox bacteria produce N2 gas by oxidizing NH4+ with NO2–, and hydroxylamine (NH2OH) is a potential intermediate of the anammox process. N2 gas production occurs when anammox bacteria are incubated with NH2OH only, indicating their capacity for NH2OH disproportionation with NH2OH serving as both the electron donor and acceptor. Limited information is currently available on NH2OH disproportionation by anammox bacteria; therefore, the stoichiometry of anammox bacterial NH2OH disproportionation was examined in the present study using 15N-tracing techniques. The anammox bacteria, Brocadia sinica, Jettenia caeni, and Scalindua sp. were incubated with the addition of 15NH2OH, and the production of 15N-labeled nitrogenous compounds was assessed. The anammox bacteria tested performed NH2OH disproportionation and produced 15-15N2 gas and NH4+ as reaction products. The addition of acetylene, an inhibitor of the anammox process, reduced the activity of NH2OH disproportionation, but not completely. The growth of B. sinica by NH2OH disproportionation (–240.3‍ ‍kJ mol NH2OH–1 under standard conditions) was also tested in 3 up-flow column anammox reactors fed with 1) 0.7‍ ‍mM NH2OH only, 2) 0.7‍ ‍mM NH2OH and 0.5‍ ‍mM NH4+, and 3) 0.7‍ ‍mM NH2OH and 0.5‍ ‍mM NO2–. NH2OH consumption activities were markedly reduced after 7‍ ‍d of operation, indicating that B. sinica was unable to maintain its activity or biomass by NH2OH disproportionation.

著者

Shiwen LIN Yawen ZHOU Weiqin ZOU Huaguo ZHANG Lin GAO Hongshu LIAO Wanchun LI

出版者

The Institute of Electronics, Information and Communication Engineers

雑誌

IEICE TRANSACTIONS on Fundamentals of Electronics, Communications and Computer Sciences (ISSN:09168508)

巻号頁・発行日

vol.E105-A, no.4, pp.673-681, 2022-04-01

被引用文献数

1

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Estimating the spatial parameters of the signals by using the effective data of a single snapshot is essential in the field of reconnaissance and confrontation. Major drawback of existing algorithms is that its constructed covariance matrix has a great degree of rank loss. The performance of existing algorithms gets degraded with low signal-to-noise ratio. In this paper, a three-parallel linear array based algorithm is proposed to achieve two-dimensional direction of arrival estimates in a single snapshot scenario. The key points of the proposed algorithm are: 1) construct three pseudo matrices with full rank and no rank loss by using the single snapshot data from the received signal model; 2) by using the rotation relation between pseudo matrices, the matched 2D-DOA is obtained with an efficient parameter matching method. Main objective of this work is on improving the angle estimation accuracy and reducing the loss of degree of freedom in single snapshot 2D-DOA estimation.

著者

Lin Gao Grant Bledsoe Hang Yin Bo Shen Lee Chao Julie Chao

出版者

日本循環器学会

雑誌

Circulation Journal (ISSN:13469843)

巻号頁・発行日

pp.CJ-12-1585, (Released:2013-05-21)

参考文献数

35

被引用文献数

5 13

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Background: Genetically modified mesenchymal stem cells (MSCs) are a promising approach to the treatment of cardiac injury after myocardial infarction (MI). Methods and Results: Rat MSCs were transduced with adenovirus containing human tissue kallikrein (TK) gene (TK-MSCs), and they secreted human TK into culture medium. Cultured TK-MSCs were more resistant to hypoxia-induced apoptosis and exhibited reduced caspase-3 activity compared to control GFP-MSCs. The effect of TK-MSC injection on cardiac injury was evaluated in rats at 1 and 14 days after MI. At 1 day after MI, human TK expression in the myocardium was associated with improved cardiac function and decreased inflammatory cell accumulation, proinflammatory gene expression and apoptosis. The beneficial effect of TK-MSCs against apoptosis was verified in cultured cardiomyocytes, as TK-MSC-conditioned medium suppressed hypoxia-induced apoptosis and caspase-3 activity, and increased Akt phosphorylation. At 2 weeks after MI, TK-MSCs improved cardiac function, decreased infarct size, attenuated cardiac remodeling, and promoted neovascularization, as compared to GFP-MSCs. Furthermore, the TK-MSC-conditioned medium, containing elevated vascular endothelial growth factor levels, stimulated the proliferation, migration and tube formation of cultured human endothelial cells. Conclusions: Our results indicate that TK-modified MSCs provide enhanced protection against cardiac injury, apoptosis and inflammation, and promote neovascularization after MI, leading to cardiac function improvement.