- 著者
- Masanori KOBAYASHI Akiko SAITO Yoshikazu TANAKA Masaki MICHISHITA Masato KOBAYASHI Mami IRIMAJIRI Takeharu KANEDA Kazuhiko OCHIAI Makoto BONKOBARA Kimimasa TAKAHASHI Tatsuya HORI Eiichi KAWAKAMI
- 出版者
- 公益社団法人 日本獣医学会
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- vol.79, no.4, pp.719-725, 2017 (Released:2017-04-05)
- 参考文献数
- 32
- 被引用文献数
- 23
Canine prostate cancer (cPCa) is an untreatable malignant neoplasm resulting in local tissue invasion and distant metastasis. MicroRNAs (miRs) are small non-coding RNAs that function as oncogenes or tumor suppressors. The purpose of this study was to characterize the expression of miRs that are altered in cPCa tissue. The expression levels of 277 mature miRs in prostatic tissue (n=5, respectively) were compared between the non-tumor and tumor groups using real-time PCR. Five miRs (miR-18a, 95, 221, 222 and 330) were up-regulated, but 14 miRs (miR-127, 148a, 205, 299, 329b, 335, 376a, 376c, 379, 380, 381, 411, 487b and 495) were down-regulated specifically in cPCa (P<0.05). These miRs have potential use as early diagnosis markers for cPCa and in miR-based therapy.
- 著者
- Masanori KOBAYASHI Akiko SAITO Yoshikazu TANAKA Masaki MICHISHITA Masato KOBAYASHI Mami IRIMAJIRI Takeharu KANEDA Kazuhiko OCHIAI Makoto BONKOBARA Kimimasa TAKAHASHI Tatsuya HORI Eiichi KAWAKAMI
- 出版者
- 公益社団法人 日本獣医学会
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- pp.16-0279, (Released:2017-02-27)
- 被引用文献数
- 23
Canine prostate cancer (cPCa) is an untreatable malignant neoplasm resulting in local tissue invasion and distant metastasis. MicroRNAs (miRs) are small non-coding RNAs that function as oncogenes or tumor suppressors. The purpose of this study was to characterize the expression of miRs that are altered in cPCa tissue. The expression levels of 277 mature miRs in prostatic tissue (n=5, respectively) were compared between the non-tumor and tumor groups using real-time PCR. Five miRs (miR-18a, 95, 221, 222 and 330) were up-regulated, but 14 miRs (miR-127, 148a, 205, 299, 329b, 335, 376a, 376c, 379, 380, 381, 411, 487b and 495) were down-regulated specifically in cPCa (P<0.05). These miRs have potential use as early diagnosis markers for cPCa and in miR-based therapy.
- 著者
- Kazuhiko OCHIAI Toshina ISHIGURO-OONUMA Yasunaga YOSHIKAWA Chihiro UDAGAWA Yuiko KATO Masami WATANABE Makoto BONKOBARA Masami MORIMATSU Toshinori OMI
- 出版者
- バイオメディカルリサーチプレス
- 雑誌
- Biomedical Research (ISSN:03886107)
- 巻号頁・発行日
- vol.36, no.2, pp.155-158, 2015-04-01 (Released:2015-04-16)
- 参考文献数
- 21
- 被引用文献数
- 2 13
Mutations in the breast cancer susceptibility gene BRCA2 leading to the failure of interactions with the recombinase RAD51 are associated with an increased risk of cancer in humans. This interaction depends on the eight BRC repeat (BRC1–8) sequences in BRCA2. We previously reported that canine BRC3 has two polymorphisms (T1425P and K1435R) influencing the interaction with RAD51, and 1435R was identified in mammary tumor dog samples. In this study, we investigated the sequence variations of BRC3 and 4 in 236 dogs of five breeds. Allele frequencies of 1425P and 1435R were 0.063 and 0.314, respectively, and there was no other polymorphism in the sequenced region. A mammalian two-hybrid assay using BRC3–4 sequences demonstrated that 1425P allele reduced the binding strength with RAD51 but 1435R had no effect. These results may provide an insight into the functions of not only individual but also multiple BRC repeats of BRCA2 in dogs.
- 著者
- Shintaro TOMURA Mona UCHIDA Tomohiro YONEZAWA Masato KOBAYASHI Makoto BONKOBARA Satoko ARAI Toru MIYAZAKI Satoshi TAMAHARA Naoaki MATSUKI
- 出版者
- 公益社団法人 日本獣医学会
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- pp.14-0166, (Released:2014-09-01)
- 被引用文献数
- 1 5
Apoptosis inhibitor of macrophage (AIM) plays roles in survival of macrophages. In this study, we cloned canine AIM cDNA and observed its transcriptional expression levels in various tissues. The coding sequence of canine AIM was 1,023 bp encoding 340 amino acid residues, which had around 65% homology with those of the human, mouse and rat. Transcriptional expression of AIM was observed in the spleen, lung, liver and lymph node, which confirmed the expression of canine AIM in tissue macrophages. Moreover, AIM was highly expressed in one of the canine histiocytic sarcoma cell lines. CD36, the receptor of AIM, was also expressed in various tissues and these cell lines. These findings are useful to reveal the actual functions of canine AIM.