著者

Sukehiro Nayu Kida Nori Umezawa Masahiro Murakami Takayuki Arai Naoko Jinnai Tsunesada Inagaki Shunichi Tsuchiya Hidetoshi Maruyama Hiroshi Tsuda Yoshio

出版者

National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

雑誌

Japanese Journal of Infectious Diseases (ISSN:13446304)

巻号頁・発行日

vol.66, no.3, pp.189-194, 2013

被引用文献数

41

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The invasion of the yellow fever mosquito <i>Aedes aegypti</i> at Narita International Airport, Japan was detected for the first time. During the course of routine vector surveillance at Narita International Airport, 27 <i>Ae. aegypti</i> adults emerged from larvae and pupae collected from a single larvitrap placed near No. 88 spot at passenger terminal 2 on August 8, 2012. After the appearance of <i>Ae. aegypti</i> in the larvitrap, we defined a 400-m buffer zone and started an intensive vector survey using an additional 34 larvitraps and 15 CO₂ traps. International aircraft and passenger terminal 2 were also inspected, and one <i>Ae. aegypti</i> male was collected from the cargo space of an international aircraft from Darwin via Manila on August 28, 2012. Larvicide treatment with 1.5% fenitrothion was conducted in 64 catch basins and one ditch in the 400-m buffer zone. Twenty-four large water tanks were also treated at least once with 0.5% pyriproxyfen, an insect growth regulator. No <i>Ae. aegypti</i> eggs or adults were found during the 1-month intensive vector survey after finding larvae and pupae in the larvitrap. We concluded that <i>Ae. aegypti</i> had failed to establish a population at Narita International Airport.

著者

Ishitsuka Yosuke Kawachi Yasuhiro Taguchi Shijima Maruyama Hiroshi Fujisawa Yasuhiro Furuta Junichi Nakamura Yasuhiro Otsuka Fujio

出版者

Nature Publishing Group

雑誌

Journal of investigative dermatology (ISSN:0022202X)

巻号頁・発行日

vol.132, no.7, pp.1775-1784, 2012-07

被引用文献数

14 4

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The epidermis is a self-renewing tissue, the homeostasis of which is dependent upon the tight balance between proliferation and differentiation based on appropriate regulation of the cell cycle. The cell cycle regulation is dependent on the interactions among a number of cell cycle regulatory molecules, including the pituitary tumor-transforming gene 1 (PTTG1), also known as securin, a regulator of sister chromatid separation and transition from metaphase to anaphase. This study was conducted to clarify the less-known functions of PTTG1 in the epidermis by the use of keratinocytes cultured under two-dimensional (2D) or three-dimensional (3D) conditions. Forced overexpression of PTTG1 caused upregulation of cyclin B1, cyclin-dependent kinase 1 (CDK1), and c-Myc, resulting in enhanced proliferation and suppression of early differentiation without apparent alterations in terminal differentiation, and the exogenous PTTG1 was downregulated in association with cell cycle exit. In contrast, depletion of PTTG1 caused their downregulation and constrained proliferation with retention of differentiation capacity. These findings suggested that PTTG1 could alter the proliferation status by modulating the expression levels of the other cell cycle regulatory proteins, and excess PTTG1 primarily affects early differentiation of keratinocytes under the stability regulation associated with cell cycle exit.