著者

Takumi Matsuzaki Masao Nakamura Takehide Nogita Atsushi Sato

出版者

The Pharmaceutical Society of Japan

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.42, no.6, pp.989-995, 2019-06-01 (Released:2019-06-01)

参考文献数

19

被引用文献数

21

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An Intact form of lactoferrin (LF) is known to be absorbed from the small intestine and transported into the blood circulation. We reevaluated the cellular uptake and release of LF using an enterocyte model of human small intestinal cells derived from the Caco-2 cell line. In contrast to a previous report, we observed that intact bovine LF was taken up into seven and 21 d-cultured Caco-2 cells and successfully released back into the culture medium, even though the human intestinal LF receptor, intelectin-1, was not immunochemically detectable. Similar observations were made for human LF and its derivatives (the N-terminal half of LF designated N-lobe and Fc fusions). These observations regarding the uptake and release of intact LF in Caco-2 cells were consistent with in vivo observations. Therefore, we propose that the uptake and release of intact LF by Caco-2 cells should be assessed as a potential in vitro model of in vivo LF absorption in human intestines.

著者

Naoshi Hirata Shiro Ohmi Shin'ichi Sakai Kei Katsumata Satoshi Matsumoto Tetsuo Takanami Akira Yamamoto Takashi Iidaka Taku Urabe Mayumi Sekine Tooru Ooida Fumihito Yamazaki Hiroshi Katao Yasuhiro Umeda Masao Nakamura Norihiko Seto Takeshi Matsushima Hiroshi Shimizu Japanese University Group of the Urgent Joint

出版者

The Seismological Society of Japan, The Volcanological Society of Japan, The Geodetic Society of Japan

雑誌

Journal of Physics of the Earth (ISSN:00223743)

巻号頁・発行日

vol.44, no.4, pp.317-328, 1996 (Released:2009-04-30)

参考文献数

21

被引用文献数

41 43

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A disastrous earthquake with a magnitude of 7.2 hit the southern part of Hyogo Prefecture on January 17, 1995. The mainshock was located on an active fault of the Arima-Takatsuki-Rokko fault system. Its focal mechanism was consistent with a right-lateral strike-slip fault trending N40°E. Three days after the occurrence of the mainshock, we started to install a highly dense seismic array in and around the fault area of the quake. Two permanent regional seismic networks of more than 30 stations covered the entire area of 200 km × 200 km. The temporarily installed array of 27 stations spanned the fault area of 15 km × 50 km. All data were telemetered to a temporary observation center at Uji. We located about 3, 100 aftershocks in real time for 1 month using an Internet connection between seismic networks. The aftershock area extended 70 km trending northeast to southwest. Hypocenters determined by the network were delivered automatically through the Internet. We found that the aftershock distribution was heterogeneous in space and time: seven clusters of hypocenters were identified and temporary variation in the rate of occurrence of aftershocks had a periodic component with periods of a half of day, 1 day, and 3 days, that are superimposed on decaying of the rate following Omori's law.

著者

Masao Nakamura Akira Shiga Ami Iimori Takumi Matsuzaki

出版者

The Pharmaceutical Society of Japan

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.46, no.7, pp.1004-1009, 2023-07-01 (Released:2023-07-01)

参考文献数

17

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Human lactoferrin (hLF) is a glycosylated globular iron-binding protein with high functional versatility that elicits anticancer, neuroprotective, and anti-inflammatory effects. Some of the diverse functions of hLF are induced after its internalization into various cells via cell surface endocytosis receptors, such as proteoglycans, which contain glycosaminoglycan (GAG) chains. We have previously demonstrated that an hLF derivative comprising the N-terminal half of hLF (referred to as the N-lobe) is internalized by intestinal enterocyte Caco-2 cells. However, the relationship between the intracellular uptake of the N-lobe and its pharmacological activity remains poorly understood. Here, we report that the N-lobe is efficiently internalized by lung cancer cells via endocytic pathways, suppressing their proliferation. Moreover, the N-lobe showed higher intracellular uptake than hLF. We found that the N-lobe was internalized into the human lung cancer cell lines PC-14 and PC-3 via clathrin- and/or caveolae-mediated endocytosis. Intracellular uptake of the N-lobe was inhibited when an equimolar concentration of chondroitin sulfate (CS)-E, a GAG subtype involved in malignant transformation and tumor metastasis, was added. The inhibitory effect of the N-lobe on PC-14 cell proliferation decreased with the addition of CS-E in a dose-dependent manner, suggesting that the CS-recognizing sequence on the N-lobe is necessary for its internalization or that the CS proteoglycan on cancer cells acts as an endocytosis receptor. These results suggest that the efficient endocytic uptake of the N-lobe is important for its antiproliferation effects on lung cancer cell lines. Thus, the N-lobe presents a promising drug candidate for cancer treatment.

著者

Masaharu MATSUMOTO Hideyuki USUI Masanori NUNAMI Masao NAKAMURA Iku SHINOHARA

出版者

The Japan Society of Plasma Science and Nuclear Fusion Research

雑誌

Plasma and Fusion Research (ISSN:18806821)

巻号頁・発行日

vol.8, pp.2406132, 2013-09-26 (Released:2015-06-13)

参考文献数

9

被引用文献数

2 3

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A parallelized two-dimensional full particle-in-cell (PIC) simulations with an adaptive mesh refinement (AMR) technique for the interaction of solar wind with kinetic scale magnetosphere around a magnetic sail are conducted. For multi-scale simulations, the newly developed AMR-PIC code has a method to modify the grid size and time step interval of computational domain in the midst of the simulations temporally and locally. In the simulation results under the typical working condition introducing the AMR technique, it is shown that the direction of interplanetary magnetic field depend strongly on the magnetospheric configuration including electron dynamics and Alfven bow shock structure. Although more validation studies are needed, the AMR-PIC code shows the worth of development for multi-scale simulations.