文献一覧: Atsushi Sato (著者)

24 0 0 0 OA Activities of daily living independence level for home discharge in stroke patients based on number of caregivers: an analysis of the Japan Rehabilitation Database

著者: Atsushi SATO Takaaki FUJITA Yuichi YAMAMOTO
出版者: Japanese Society of Physical Therapy
雑誌: Physical Therapy Research (ISSN:21898448)
巻号頁・発行日: pp.E9914, (Released:2017-07-28)
参考文献数: 26
被引用文献数: 6 7

Purpose: This study aimed to calculate cut-off values of activities of daily living independence level for stroke patient home discharge based on the number of family caregivers. Method: The subjects comprised 1442 stroke patients (26 hospitals) who were registered of the Japanese Rehabilitation Database. Receiver operating characteristic curves were used to elucidate the BI and FIM® instrument scores necessary for home discharge. Analysis was performed for each subject according to the number of family caregivers, i.e., no caregiver, one person, two persons or more, and overall. Result: The BI cut-off points that discriminated between home discharge and other were 65/60 points overall, 75/70 points in patients with no caregiver, 65/60 points in patients with one caregiver, and 60/55 points in patients with two or more caregivers. The FIM® instrument cut-off points were 90/89 points overall, 101/100 points in patients with no caregiver, 87/86 points in patients with one caregiver, and 87/86 points in patients with two or more caregivers. Conclusion: Our results indicated that home discharge for patients with many caregivers was possible even with low ADL independence levels, and that there was a large difference in cut-off values depending on the presence or absence of one caregiver.

2023-05-16 20:31:42
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4 0 0 0 OA Cellular Uptake and Release of Intact Lactoferrin and Its Derivatives in an Intestinal Enterocyte Model of Caco-2 Cells

著者: Takumi Matsuzaki Masao Nakamura Takehide Nogita Atsushi Sato
出版者: The Pharmaceutical Society of Japan
雑誌: Biological and Pharmaceutical Bulletin (ISSN:09186158)
巻号頁・発行日: vol.42, no.6, pp.989-995, 2019-06-01 (Released:2019-06-01)
参考文献数: 19
被引用文献数: 21

An Intact form of lactoferrin (LF) is known to be absorbed from the small intestine and transported into the blood circulation. We reevaluated the cellular uptake and release of LF using an enterocyte model of human small intestinal cells derived from the Caco-2 cell line. In contrast to a previous report, we observed that intact bovine LF was taken up into seven and 21 d-cultured Caco-2 cells and successfully released back into the culture medium, even though the human intestinal LF receptor, intelectin-1, was not immunochemically detectable. Similar observations were made for human LF and its derivatives (the N-terminal half of LF designated N-lobe and Fc fusions). These observations regarding the uptake and release of intact LF in Caco-2 cells were consistent with in vivo observations. Therefore, we propose that the uptake and release of intact LF by Caco-2 cells should be assessed as a potential in vitro model of in vivo LF absorption in human intestines.

2019-08-01 21:12:47
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2 0 0 0 OA Feasibility Study for Future Space-Borne Coherent Doppler Wind Lidar, Part 1: Instrumental Overview for Global Wind Profile Observation

著者: Shoken ISHII Philippe BARON Makoto AOKI Kohei MIZUTANI Motoaki YASUI Satoshi OCHIAI Atsushi SATO Yohei SATOH Takuji KUBOTA Daisuke SAKAIZAWA Riko OKI Kozo OKAMOTO Toshiyuki ISHIBASHI Taichu Y. TANAKA Tsuyoshi T. SEKIYAMA Takashi MAKI Koji YAMASHITA Tomoaki NISHIZAWA Masaki SATOH Toshiki IWASAKI
出版者: (公社)日本気象学会
雑誌: 気象集誌. 第2輯 (ISSN:00261165)
巻号頁・発行日: vol.95, no.5, pp.301-317, 2017 (Released:2017-10-04)
参考文献数: 57
被引用文献数: 15

A working group is studying the feasibility of a future Japanese space-borne coherent Doppler wind lidar (CDWL) for global wind profile observation. This study is composed of two companion papers: an instrumental overview of the space-borne CDWL for global wind profile observation (Part 1), and the wind measurement performance (error and bias) investigated using a full-fledged space-borne CDWL simulator (Part 2). This paper aims to describe the future space-borne CDWL in terms of technical points and observation user requirements. The future mission concept is designed to have two looks for vector wind measurement with vertical resolutions of 0.5 (lower troposphere: 0-3 km), 1 (middle troposphere: 3-8 km), and 2 km (upper troposphere: 8-20 km) and horizontal resolution of < 100 km along a satellite. The altitude and orbit of the satellite are discussed from a scientific viewpoint. The candidate altitude and orbit of the satellite are 220 km and an inclination angle of 96.4° (polar orbit) or 35.1° (low-inclination-angle orbit). The technical requirements of the space-borne CDWL are a single-frequency 2-μm pulse laser with an average laser power of 3.75 W, two effective 40-cm-diameter afocal telescopes, a wide-bandwidth (> 3.4 GHz) detector, a high-speed analog-to-digital converter, and a systematic lidar efficiency of 0.08. The space-borne CDWL looks at two locations at a nadir angle of 35° at two azimuth angles of 45° and 135° (225° and 315°) along the satellite track. The future space-borne CDWL wind profile observation will fill the gap of the current global wind observing systems and contribute to the improvement of the initial conditions for numerical weather prediction (NWP), the prediction of typhoons and heavy rain, and various meteorological studies.

2017-10-06 09:34:28
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1 0 0 0 OA Improved Refolding of a Human IgG1 Fc (CH2-CH3) Scaffold from Its Inclusion Body in E. coli by Alkaline Solubilization

著者: Show Ishikawa Haruna Ishikawa Atsushi Sato
出版者: The Pharmaceutical Society of Japan
雑誌: Biological and Pharmaceutical Bulletin (ISSN:09186158)
巻号頁・発行日: vol.45, no.3, pp.284-291, 2022-03-01 (Released:2022-03-01)
参考文献数: 27
被引用文献数: 2

Recently, we developed a platform strategy for hinge-deficient human immunoglobulin G1 (IgG1) Fc fusion as a non-immunostimulatory Fc fusion system. As a starting point to establish a promising approach for generating hinge-deficient Fc fusion proteins in Escherichia (E.) coli, we selected a CH2-CH3 scaffold as a model protein for evaluation. Recombinant CH2-CH3, expressed as inclusion bodies, was solubilized with various denaturants (urea, sarkosyl, sodium dodecyl sulfate (SDS), 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), or Triton X-100) in neutral (phosphate-buffered saline (PBS), pH 8) or alkaline (50 or 500 mM N-cyclohexyl-3-aminopropanesulfonic acid (CAPS), pH 11) buffer at 25 °C. Similar to the authentic CH2-CH3 produced in Chinese hamster ovary (CHO) cells, all denaturants, except urea in CAPS buffer but not in PBS, were found to elicit the dimer formation of solubilized CH2-CH3 on SDS-polyacrylamide gel electrophoresis (PAGE). After dialysis with PBS, sarkosyl-soluble CH2-CH3 inclusion bodies were successfully purified using protein G-Sepharose, indicating their successful refolding. Compared to the purified CH2-CH3 from its sarkosyl-soluble inclusion bodies in neutral buffer, that in 500 mM CAPS alkaline buffer revealed substantial structure-related similarities, such as secondary structures and thermal stabilities, as measured by circular dichroism spectroscopy, to authentic CH2-CH3. Native PAGE analysis also supported the above data. Therefore, solubilization at alkaline pH is an essential factor that promotes the refolding of CH2-CH3. Dimer formation of CH2-CH3 on SDS-PAGE may act as a surrogate marker for its protein refolding status. Our observations may provide important hints toward downstream processing of Fc-fusion production in E. coli.

2022-03-03 15:55:49
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1 0 0 0 OA Concurrent and discriminant validity of Nintendo Wii Fit exergame for the assessment of postural sway

著者: Atsushi Sato Ah-Cheng Goh
出版者: The Society of Physical Therapy Science
雑誌: Journal of Physical Therapy Science (ISSN:09155287)
巻号頁・発行日: vol.33, no.2, pp.100-105, 2021 (Released:2021-02-13)
参考文献数: 30
被引用文献数: 4

[Purpose] The purpose of this study was to determine the concurrent validity of Wii Fit center-of-pressure parameters with Clinical Test of Sensory Integration for Balance and to evaluate the discriminant validity of the Wii Fit center-of-pressure parameters for adults and the elderly for the assessment of postural sway. [Participants and Methods] This study used an observational cross-sectional correlational design. All 70 participants were required to complete a questionnaire for eligibility screening, followed by a center-of-pressure assessment using three Wii Fit balance activities (snowboard slalom, ski slalom, and balance bubble) and Clinical Test of Sensory Integration for Balance using a force platform. [Results] For center-of-pressure assessment, our results showed there was concurrent validity of Wii Fit ski slalom and balance bubble with Clinical Test of Sensory Integration for Balance conditions 6 and 4, respectively. Our results also demonstrated that the three Wii Fit balance activities selected in this study could be used to discriminate between adults and the elderly. [Conclusion] Our results suggest that Wii Fit is a viable and affordable alternative method for center-of-pressure assessment.

2021-02-18 08:45:26
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1 0 0 0 OA Evaluation of a chest rehabilitation project in Nepal using the St. George’s Respiratory Questionnaire and Chronic Obstructive Pulmonary Disease Assessment Test

著者: Atsushi Sato Mitsuhiro Kamimura Keisuke Yorimoto Taro Kato Shohei Yamashita Atsuto Mouri Motoaki Tanigawa Yoshihito Arimoto Junko Fujitani Karbir Nath Yogi Khem Bahadur Karki Shigeki Hayashi
出版者: The Society of Physical Therapy Science
雑誌: Journal of Physical Therapy Science (ISSN:09155287)
巻号頁・発行日: vol.32, no.12, pp.795-799, 2020 (Released:2020-12-11)
参考文献数: 15
被引用文献数: 1

[Purpose] The incidence of chronic obstructive pulmonary disease is rapidly increasing worldwide. In Nepal, it has the highest mortality rate among all noninfectious diseases. Since 2015, we have been involved in a project that aims to facilitate chest rehabilitation for patients with chronic obstructive pulmonary disease in Nepal. We compared the Nepali version of the St. George’s Respiratory Questionnaire with the Chronic Obstructive Pulmonary Disease Assessment Test, the latter of which was translated into Nepali for this project. We also evaluated the extent to which patient quality of life improved after the rehabilitation program. [Participants and Methods] The Nepali St. George’s Respiratory Questionnaire and Chronic Obstructive Pulmonary Disease Assessment Test were used to assess the health status of patients both before the intervention’s initiation and one year after it. Between May and September of 2016, 122 patients with chronic obstructive pulmonary disease participated in this program. [Results] We collected valid responses from 57 patients both before and after the intervention. The scores of both screening tools were significantly lower after the intervention than before and showed a significant correlation with one another. [Conclusion] These results suggest that the Nepali version of the Chronic Obstructive Pulmonary Disease Assessment Test is a reliable tool for the evaluation of chronic obstructive pulmonary disease and that the intervention used in the project might be effective for patients afflicted with the disease. However, there are limitations to the research design, such as the limited number of participants used in the study.

2021-01-08 04:50:03
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1 0 0 0 OA The pain-relieving effects of lactoferrin on oxaliplatin-induced neuropathic pain

著者: Takeshi FUJIMURA Aiko IGUCHI Atsushi SATO Shinji KAGAYA Tatsuo HOSHINO Takashi TAKEUCHI
出版者: JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌: Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日: pp.20-0034, (Released:2020-09-25)
被引用文献数: 1

Oxaliplatin (OXL) therapy often causes side effects including chronic peripheral neuropathy. We investigated the pain-relieving effects of recombinant human lactoferrin (rhLf) as well as a long-acting IgG-Fc fused rhLf (rhLf-Fc) on OXL-induced neuropathic pain. We used the hLf in this study, because the homology between mouse Lf and hLf is higher than that of bovine Lf. In addition, rhLf-Fc is expected to enhance the analgesic effect due to the life extension effect in the body. We administered OXL (2 mg/kg, i.v.) to mice twice weekly for 4 weeks. Phosphate buffered saline (PBS), rhLf (100 mg/kg, i.p.) or rhLf-Fc (100 mg/kg, i.p.) was administered once a week from day 15 to 32. We also assessed the continuous infusion of same drugs (10 mg/kg/day) into the external jugular vein by using an osmotic pump. Both of rhLf and rhLf-Fc significantly reduced the hypersensitivity to mechanical stimulation when they were administered intraperitoneally. The continuous infusion of rhLf resulted in a more pronounced effect. Histopathological analysis of sciatic nerve showed that both rhLf and rhLf-Fc tended to reduce nerve fiber damage, but no significant difference was observed in nerve fiber cross-sectional area. Therefore, it was suggested that rhLf or rhLf-Fc injection could be an option for controlling neuropathic pain, which are side effects of OXL.

2020-09-30 02:06:40
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1 0 0 0 OA Hydrolyzed diets may stimulate food-reactive lymphocytes in dogs

著者: Kenichi MASUDA Atsushi SATO Atsushi TANAKA Akiko KUMAGAI
出版者: JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌: Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日: vol.82, no.2, pp.177-183, 2020 (Released:2020-02-18)
参考文献数: 31
被引用文献数: 4

Hydrolyzed proteins are often prescribed for dogs with food hypersensitivity in food elimination programs. However, the potential of these diets to stimulate lymphocyte-mediated hypersensitivity is currently unknown. In this study, two commercially available hydrolyzed diets for dogs, D-1 (Aminopeptide Formula Dry, Royal Canin Japon, Tokyo, Japan), and D-2 (Canine z/d Ultra Dry, Hill’s-Colgate (Japan) Ltd., Tokyo, Japan), were analyzed to identify residual proteins or peptides, as well as activated helper T-lymphocyte reactions in dogs with suspected food hypersensitivity. Proteins and peptides with molecular weights >1 kDa (majority 1.5–3.5 kDa) were detected in both diet extracts with sodium dodecyl sulfate polyacrylamide gel electrophoresis, and size exclusion chromatography. When peripheral blood mononuclear cells (PBMC’s) from 316 dogs with suspected food allergies were cultured with hydrolyzed diet extracts, flow cytometry analysis revealed detectable levels of CD25low helper T-lymphocytes stimulated by D-1 and D-2 in 91 of 316, (28.8%), and 75 of 316 (23.7%) samples, respectively. These data indicated that the extracts contained proteins or peptides large enough to activate the lymphocytes. The percentages of CD25low helper T-lymphocytes stimulated by D-1 and D-2 extracts increased to 38.7% and 29.6%, respectively, in 186 of the original 316 samples (186/316, 58.9%), also reactive to poultry-related antigens. Thus, both poultry-related antigens, and D-1 and D-2 diet extracts may activate helper T-lymphocytes. These results demonstrate that hydrolyzed diets may contain proteins that stimulate helper T-lymphocytes, and may not be effective for treating all dogs with food hypersensitivity.

2020-06-12 17:49:03
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1 0 0 0 OA Hydrolyzed diets may stimulate food-reactive lymphocytes in dogs

著者: Kenichi MASUDA Atsushi SATO Atsushi TANAKA Akiko KUMAGAI
出版者: JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌: Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日: pp.19-0222, (Released:2019-12-25)
被引用文献数: 4

2019-12-31 19:52:43
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