著者

松本 健 Matsumoto Ken

出版者

筑波大学比較・理論文学会

雑誌

文学研究論集 (ISSN:09158944)

巻号頁・発行日

no.23, pp.46(17)-62(1), 2005-03-31

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一、 はじめに 「浮世物語」をめぐる先行研究の多くは、前半から後半への流れにおいて見られる様相の変化に注目している。「浮世物語」ははじめ、矮小滑稽に設定された主人公の批判されるべき振る舞いを描くハナシが展開されているのだが、 ...

著者

Monden Akito Nakae Daikai Kamiya Toshihito Sato Shin-ichi Matsumoto Ken-ichi

出版者

Nara Institute of Science and Technology

巻号頁・発行日

2001-09

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Existing researches suggest that the code clone (duplicated code) is one of the factors that degrades the design and structure of software and lowers the software quality such as readability and maintainability. However, the influence of code clones on software quality has not been quantitatively clarified yet. In this paper we tried to quantitatively clarify the relation between code clones and the software reliability and maintainability of twenty years old software. As a result, we found that modules having a code clone (clone-included modules) are more reliable than modules having no code clone (non-clone modules) in average. Nevertheless, the modules having very large code clones (more than 200 lines) are less reliable than non-clone modules. We also found that clone-included modules are less maintainable than non-clone modules; and, modules having larger code clone are less maintainable than modules having smaller code clone.

著者

Kadoya Ryosuke Matsumoto Ken'ichiro Ooi Toshihiko Taguchi Seiichi

出版者

PLOS

雑誌

PLOS one (ISSN:19326203)

巻号頁・発行日

vol.10, no.6, 2015-06-04

被引用文献数

19

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Bacterial polyester polyhydroxyalkanoates (PHAs) have been produced in engineered Escherichia coli, which turned into an efficient and versatile platform by applying metabolic and enzyme engineering approaches. The present study aimed at drawing out the latent potential of this organism using genome-wide mutagenesis. To meet this goal, a transposon-based mutagenesis was carried out on E. coli, which was transformed to produce poly (lactate-co-3-hydroxybutyrate) from glucose. A high-throughput screening of polymer-accumulating cells on Nile red-containing plates isolated one mutant that produced 1.8-fold higher quantity of polymer without severe disadvantages in the cell growth and monomer composition of the polymer. The transposon was inserted into the locus within the gene encoding MtgA that takes part, as a non-lethal component, in the formation of the peptidoglycan backbone. Accordingly, the mtgA-deleted strain E. coli JW3175, which was a derivate of superior PHA-producing strain BW25113, was examined for polymer production, and exhibited an enhanced accumulation of the polymer (7.0 g/l) compared to the control (5.2 g/l). Interestingly, an enlargement in cell width associated with polymer accumulation was observed in this strain, resulting in a 1.6-fold greater polymer accumulation per cell compared to the control. This result suggests that the increase in volumetric capacity for accumulating intracellular material contributed to the enhanced polymer production. The mtgA deletion should be combined with conventional engineering approaches, and thus, is a promising strategy for improved production of intracellularly accumulated biopolymers.

著者

Nomura Jun Matsumoto Ken-Ichi Iguchi-Ariga Sanae M M Ariga Hiroyoshi

出版者

Spandidos Publications

雑誌

Oncology reports (ISSN:1021335X)

巻号頁・発行日

vol.14, no.5, pp.1305-1309, 2005-11

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Fas-mediated apoptosis has been proposed to play an important role in homeostasis. Fas triggers apoptosis after stimulation by its ligand FasL or the Fas ligand agonist anti-Fas antibody through a mitochondria-dependent or -independent pathway, and MSSP has been identified as a transcription factor that regulates the c-myc gene and was later found to positively or negatively regulate a variety of genes, including alpha-smooth actin, MHC class I, MHC class 2 and the thyrotropin receptor. We further found that expression of the Fas gene was repressed, resulting in abrogation of the Fas-mediated induction of apoptosis both in Mssp-knockout mice and primary thymocytes. MSSP was then found to stimulate promoter activity of the Fas gene by binding to a specific region. In this study, to identify the MSSP-dependent Fas-induced apoptosis pathway, primary fibroblasts from MSSP (+/+) and MSSP (-/-) cells were treated with the combination of interleukin 1-beta and interferon-gamma and expression of the Fas gene was examined. The results showed that the Fas gene was expressed at the same levels in the two cell types. Furthermore, when these cells were treated with the anti-Fas antibody, it was found that cytochrome C was not released in the cytosol and that activations of caspase 8 and caspase 3 occurred in primary fibroblasts from MSSP (+/+) cells but not from MSSP (-/-) cells. These results indicate that Fas-mediated apoptosis induced by MSSP occurs independently of mitochondria.