- 著者
- Sei Harada Miho Iida Naoko Miyagawa Aya Hirata Kazuyo Kuwabara Minako Matsumoto Tomonori Okamura Shun Edagawa Yoko Kawada Atsuko Miyake Ryota Toki Miki Akiyama Atsuki Kawai Daisuke Sugiyama Yasunori Sato Ryo Takemura Kota Fukai Yoshiki Ishibashi Suzuka Kato Ayako Kurihara Mizuki Sata Takuma Shibuki Ayano Takeuchi Shun Kohsaka Mitsuaki Sawano Satoshi Shoji Yoshikane Izawa Masahiro Katsumata Koichi Oki Shinichi Takahashi Tsubasa Takizawa Hiroshi Maruya Yuji Nishiwaki Ryo Kawasaki Akiyoshi Hirayama Takamasa Ishikawa Rintaro Saito Asako Sato Tomoyoshi Soga Masahiro Sugimoto Masaru Tomita Shohei Komaki Hideki Ohmomo Kanako Ono Yayoi Otsuka-Yamasaki Atsushi Shimizu Yoichi Sutoh Atsushi Hozawa Kengo Kinoshita Seizo Koshiba Kazuki Kumada Soichi Ogishima Mika Sakurai-Yageta Gen Tamiya Toru Takebayashi
- 出版者
- Japan Epidemiological Association
- 雑誌
- Journal of Epidemiology (ISSN:09175040)
- 巻号頁・発行日
- pp.JE20230192, (Released:2024-01-06)
- 参考文献数
- 40
- 被引用文献数
- 1
The Tsuruoka Metabolomics Cohort Study (TMCS) is an ongoing population-based cohort study being conducted in the rural area of Yamagata Prefecture, Japan. This study aimed to enhance the precision prevention of multi-factorial, complex diseases, including non-communicable and aging-associated diseases, by improving risk stratification and prediction measures. At baseline, 11,002 participants aged 35–74 years were recruited in Tsuruoka City, Yamagata Prefecture, Japan, between 2012 and 2015, with an ongoing follow-up survey. Participants underwent various measurements, examinations, tests, and questionnaires on their health, lifestyle, and social factors. This study used an integrative approach with deep molecular profiling to identify potential biomarkers linked to phenotypes that underpin disease pathophysiology and provide better mechanistic insights into social health determinants. The TMCS incorporates multi-omics data, including genetic and metabolomic analyses of 10,933 participants and comprehensive data collection ranging from physical, psychological, behavioral, and social to biological data. The metabolome is used as a phenotypic probe because it is sensitive to changes in physiological and external conditions. The TMCS focuses on collecting outcomes for cardiovascular disease, cancer incidence and mortality, disability, functional decline due to aging and disease sequelae, and the variation in health status within the body represented by omics analysis that lies between exposure and disease. It contains several sub-studies on aging, heated tobacco products, and women's health. This study is notable for its robust design, high participation rate (89%), and long-term repeated surveys. Moreover, it contributes to precision prevention in Japan and East Asia as a well-established multi-omics platform.
- 著者
- Eiji Soga Miki Akiyama Yusuke Ohsaki Wataru Hayashi Takehisa Matsumoto Kozue Oana Noriyuki Nagano Yoshiyuki Kawakami
- 出版者
- National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee
- 雑誌
- Japanese Journal of Infectious Diseases (ISSN:13446304)
- 巻号頁・発行日
- vol.72, no.3, pp.193-195, 2019 (Released:2019-05-23)
- 参考文献数
- 11
- 被引用文献数
- 4
A capnophilic Gram-negative rod-shaped bacterium was recovered from the urine of an octogenarian male patient with acute pyelonephritis. The isolate was found to produce CTX-M-2-type extended-spectrum β-lactamase. Interestingly, the isolate failed to grow on modified Drigalski (BTB) and MacConkey agar media, even under CO2-enriched atmosphere. Our analysis revealed that the pH-indicator dyes, bromothymol blue, and/or crystal violet that were incorporated into the agar media inhibited the growth of the isolate. Although routine identification methods using Vitek® 2 Compact systems were unsuccessful, the isolate was identified as Proteus mirabilis by 16S rRNA sequencing and MALDI-TOF MS analysis. The carbonic anhydrase (CA) region spanning approximately 2,000 bp upstream to 2,000 bp downstream, which is responsible for the CO2 requirement, was not amplified, which could be attributed to the large-scale deletion or mutation of the DNA sequences containing the CA gene region. In fact, revertants with the ability to grow without CO2 were not detected. However, a revertant that was capable of growing in both BTB and MacConkey agar was detected at frequencies less than 10-9. Therefore, the genes responsible for the highly sensitive reactions of the isolate to pH indicator dyes is not likely to be linked to the CA genes.