著者

Yihan Wang Yang Liu Jieting Liu Min Wang Yingbin Wang

出版者

The Pharmaceutical Society of Japan

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.45, no.1, pp.27-33, 2022-01-01 (Released:2022-01-01)

参考文献数

40

被引用文献数

1

---

This study aimed to explore the effect of curcumin and hydromorphone hydrochloride (HH) cotreatment on postoperative pain in rats. An incision + formaldehyde-induced pain rat model was established. Rats were treated with vehicle, curcumin, HH, or curcumin + HH. Paw mechanical withdrawal threshold and thermal withdrawal latency were measured at 1 d before surgery as well as 1 , 2 h, 1 , 3 , and 7 d after surgery to assess pain sensitivity. The L4-6 region of the spinal cord was collected from each rat at 2 h, 1 , 3 , and 7 d after surgery. Western blot analysis and immunohistochemical staining were carried out to detect the protein expression of pain-related genes. Quantitative real-time PCR and enzyme-linked immunosorbent assay were conducted to measure the expression and production of proinflammatory mediators. Compared with other groups, Curcumin + HH significantly reduced pain sensitivity in the model rats. Mechanistically, curcumin + HH suppressed protein expression of stromal cell-derived factor-1 (SDF-1), CXC chemokine receptor 4 (CXCR4), p-Akt, and c-fos while enhancing protein expression of nerve growth factor (NGF) in the dorsal root ganglia (DRG) of model rats. Curcumin + HH inhibited the expression and production of interleukin 1β (IL-1β), cyclooxygenase-2 (COX-2), tumor necrosis factor α (TNF-α), and p65 nuclear factor kappa B (NF-κB) in the DRG. Coadministration of curcumin and HH alleviates incision + formaldehyde-induced pain in rats, possibly by suppressing the SDF-1/CXCR4 pathway and the production of proinflammatory mediators. Our results provide curcumin and HH cotreatment as a promising therapeutic strategy in the management of postoperative pain.

著者

Yan Gao Chuanqi Zhang Yisi Liu Min Wang

出版者

International Research and Cooperation Association for Bio & Socio-Sciences Advancement

雑誌

BioScience Trends (ISSN:18817815)

巻号頁・発行日

pp.2019.01021, (Released:2019-04-24)

参考文献数

27

被引用文献数

30

---

Circular RNA (circRNA) is a class of endogenous non-coding RNAs that are closely related to the pathogenesis of many human diseases, particularly cancer. However, the characterization of circRNAs in high-grade serous ovarian cancer (HGSOC) remains unknown. This study aimed to investigate the expression profile of circRNAs in HGSOC. Expression profiles of circRNAs differential expression based on circRNAs High-throughput sequencing were identified in 3 HGSOC specimens and 3 normal ovarian tissues. A total of 710 differentially expressed circRNAs were found (354 expressions up-regulated and 356 expressions down-regulated). CircRNA sequencing data were verified by qRT-PCR in HGSOC tissue and benign ovarian lesions. Differential expression of 7 circRNAs (circRNA385, circRNA2058, circRNA3336, circRNA2606, circRNA1656, circRNA1312 and circRNA7474) in HGSOC tissue was confirmed by qRT-PCR. Among them, circRNA1656 showed the highest fold change. qRT-PCR was used to verify the expression of circRNA1656 in ovarian cancer cell lines. In order to analyze the relationship between circRNA1656 expression and clinical pathological biological characteristics of HGSOC, qRT-PCR was used to verify the expression of circRNA1656 in 60 HGSOC tissues compared with 60 benign ovarian lesions. The expression of circRNA1656 was down-regulated in HGSOC tissues and ovarian cancer cell lines, and correlated with the FIGO stage of HGSOC. circRNA1656 has the potential to serve as a novel tumor marker for HGSOC.

著者

Xun CHEN Xue-nong DUAN Li-min WANG Yi-ping CHEN Dun-dun WANG

出版者

The Japan Society of Mechanical Engineers

雑誌

Proceedings of the International Conference on Power Engineering : ICOPE (ISSN:24242942)

巻号頁・発行日

pp._ICOPE-15--_ICOPE-15-, 2015-11-30 (Released:2017-06-19)

---

Under the circumstances of different inlet air heating modes for thermal units with rotary air preheaters using flue gas denitrification, in order to perform the comparison and analysis on the outlet gas and air temperatures, the wall temperature of the heat storage element, and the influencing trend and variation patterns of acid corrosion area and ammonium bisulfate deposition area, the thermodynamic properties of the air pre-heater are calculated. Based on the thermodynamic model, the calculation are done on the air pre-heater of a 600MW power plant, with hot air recirculation mode and inlet air heating mode respectively. The results show that in the hot air recirculation mode, with the hot air recirculation ratio increasing, the average outlet wall temperature of the cold-section goes up, while that of the hot-section goes down. Meanwhile, with the acid corrosion area in the cold-section shrinking, the ammonium bisulfate deposition area ascends, which intensifies ash clogging in the air pre-heater. In the steam air heating mode, the average outlet wall temperature of the heat storage elements at both the hot and cold section increase, and the acid corrosion and ammonium bisulfate buildup areas descend simultaneously. Thus, for the air heater of thermal power units using flue gas denitrification technology and high-sulphur coal, the calculations for thermal check should be conducted for winter peak load scenarios, and the inlet air heating by steam air heater is more preferable to be applied in practice.

著者

Xun CHEN Xue-nong DUAN Li-min WANG

出版者

The Japan Society of Mechanical Engineers

雑誌

Proceedings of the International Conference on Power Engineering : ICOPE (ISSN:24242942)

巻号頁・発行日

pp._ICOPE-15--_ICOPE-15-, 2015-11-30 (Released:2017-06-19)

---

The heat transfer performance of a tri-sector rotary air pre-heater was comprehensively investigated considering the influences of coal quality, flue gas flow rate, ambient temperature and load variation. By applying the standard of ASME PTC 4.3 with actual characteristics of the tri-sector rotary air preheater, an applicable index evaluation system for the tri-sector rotary air preheater was established. Combining the energy conservation principle and the parameters matching relationship of thermal systems, test methods of the corresponding key parameters were introduced. Finally, a typical application of the index evaluation system in the analysis and evaluation of the thermal performance and heat transfer effect of a retrofitted tri-sector rotary air preheater for a 300 MW unit was introduced.