著者
Fumi Tatsuzawa Kenjiro Toki Yuko Ohtani Kazuhisa Kato Norio Saito Toshio Honda Masahiro Mii
出版者
園芸学会
雑誌
Journal of the Japanese Society for Horticultural Science (ISSN:18823351)
巻号頁・発行日
pp.CH-100, (Released:2014-04-26)
被引用文献数
2 7

Two anthocyanins (pigments 1 and 2) were detected from the blue flowers of Nemophila menziesii ‘Insignis blue’ and the purple flowers of its variants as the main floral anthocyanins. These two anthocyanins were isolated from the blue flowers and elucidated to be petunidin 3-O-[6-O-(cis-p-coumaroyl)-β-glucopyranoside]-5-O-[6-O-(malonyl)-β-glucopyranoside] (1) and petunidin 3-O-[6-O-(trans-p-coumaroyl)-β-glucopyranoside]-5-O-[6-O-(malonyl)-β-glucopyranoside] (2), respectively, by chemical and spectroscopic means, and pigment 1 was confirmed as a new anthocyanin in plants. Two flavonol glycosides (pigments 3 and 5) and two flavone glycosides (pigments 4 and 6) were also isolated from the blue flowers, and were identified to be kaempferol 3-(6-rhamnosyl)-glucoside-7-glucoside (3), apigenin 7,4′-di-glucoside (4), kaempferol 3-(2,6-di-rhamnosyl)-glucoside (5), and apigenin 7-glucoside-4′-(6-malonyl)-glucoside (6) as major flavonoids. Among these four flavonoids, however, pigments 4 and 6 (flavones) were not detected in the purple flowers. These results might be attributed to color production in blue and purple flowers.
著者
Norikazu MIYASHITA Yasuaki KUBO Miharu YONAI Kanako KANEYAMA Norio SAITO Ken SAWAI Akira MINAMIHASHI Toshiyuki SUZUKI Toshiyuki KOJIMA Takashi NAGAI
出版者
THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT
雑誌
Journal of Reproduction and Development (ISSN:09168818)
巻号頁・発行日
pp.1108020393-1108020393, (Released:2011-08-05)
被引用文献数
6 13

Dolly, the first mammal cloned from a somatic cell, had shorter telomeres than age-matched controls and died at an early age because of disease. To investigate longevity and lifetime performance in cloned animals, we produced cloned cows with short telomeres using oviductal epithelial cells as donor cells. At 5 years of age, despite the presence of short telomeres, all cloned cows delivered multiple healthy offspring following artificial insemination with conventionally processed spermatozoa from noncloned bulls, and their milk production was comparable to that of donor cows. Moreover, this study revealed that the offspring had normal-length telomeres in their leukocytes and major organs. Thus, cloned animals have normal functional germ lines, and therefore germ line function can completely restore telomere lengths in clone gametes by telomerase activity, resulting in healthy offspring with normal-length telomeres.