著者

Kasahara Masahiro Yashiro Kumiko Sakamoto Toshio Ohmori Masayuki

出版者

Japanese Society of Plant Physiologists

雑誌

Plant and cell physiology (ISSN:00320781)

巻号頁・発行日

vol.38, no.7, pp.828-836, 1997

参考文献数

48

被引用文献数

3

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A cyaC gene encoding an adenylate cyclase of the filamentous cyanobacterium Spirulina platensis was sequenced. The predicted amino acid sequence of the C-terminal region of cyaC is similar to the catalytic domains of adenylate cyclases in other cyanobacteria and eukaryotes. The sequences of other regions are similar to those of proteins consisting of the bacterial two-component signal transduction system: the sensory kinase and the response regulator. The predicted gene product of cyaC contains, from the N-terminal end, a receiver domain of the response regulator protein (R1), a domain similar to the ETR1 of Arabidopsis thaliana, a transmitter domain of the sensory kinase protein, a receiver domain of the response regulator protein (R2), and a catalytic domain of adenylate cyclase. The cyaC gene was expressed as an affinity-tagged protein in Escherichia coli, and the recombinant protein was purified. The purified protein had adenylate cyclase activity which was activated by Mn^<2+>. The results of Western blotting using an anti-CyaC antiserum and the S. platensis cell extract confirmed that cyaC gene is expressed in S. platensis.

著者

Ohmori Masayuki Okamoto Shinobu Fujisawa Takatomo

出版者

一般社団法人日本植物生理学会

雑誌

Plant and cell physiology (ISSN:00320781)

巻号頁・発行日

vol.42, 2001