著者

Kasahara Masahiro Yashiro Kumiko Sakamoto Toshio Ohmori Masayuki

出版者

Japanese Society of Plant Physiologists

雑誌

Plant and cell physiology (ISSN:00320781)

巻号頁・発行日

vol.38, no.7, pp.828-836, 1997

参考文献数

48

被引用文献数

3

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A cyaC gene encoding an adenylate cyclase of the filamentous cyanobacterium Spirulina platensis was sequenced. The predicted amino acid sequence of the C-terminal region of cyaC is similar to the catalytic domains of adenylate cyclases in other cyanobacteria and eukaryotes. The sequences of other regions are similar to those of proteins consisting of the bacterial two-component signal transduction system: the sensory kinase and the response regulator. The predicted gene product of cyaC contains, from the N-terminal end, a receiver domain of the response regulator protein (R1), a domain similar to the ETR1 of Arabidopsis thaliana, a transmitter domain of the sensory kinase protein, a receiver domain of the response regulator protein (R2), and a catalytic domain of adenylate cyclase. The cyaC gene was expressed as an affinity-tagged protein in Escherichia coli, and the recombinant protein was purified. The purified protein had adenylate cyclase activity which was activated by Mn^<2+>. The results of Western blotting using an anti-CyaC antiserum and the S. platensis cell extract confirmed that cyaC gene is expressed in S. platensis.

著者

Sakamoto Toshio

出版者

一般社団法人日本物理学会

雑誌

Progress of theoretical physics (ISSN:0033068X)

巻号頁・発行日

vol.78, no.2, pp.423-434, 1987-08-25

被引用文献数

1

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In the method of stochastic quantization proposed by Parisi and Wu, the Langevin equation is set by using an additional fictitious time. The results of the usual quantization are obtained by taking the infinite limit of the time. For a system with a special class of potential, however, it is also possible to formulate the stochastic quantization based on the Langevin equation associated with the ordinary time. Applying this method, we investigate the stochastic quantization of the open-ends Nambu-Goto string. It is shown that the effective action derived out of the Langevin equation of the string has the form of a supersymmetric string, though the fermionic degrees of freedom are ghost variables.