著者

Yanagawa,Hiroshi

出版者

Japanese Society of Plant Physiologists

雑誌

Plant and cell physiology

巻号頁・発行日

vol.14, no.4, 1973-08

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Plant growth inhibitors I, II, III, VII, VIII, which occur in asparagus, promoted growth and pyruvate oxidation in Streptococcus faecalis 10Cl. Activities were compared with those obtained withα-lipoic acid and several structurally related synthetic sulfur containing compounds.

著者

MORITA Shimpei FUKASE Masami HOSHINO Kumiko FUKUDA Yoichi YAMAGUCHI Masami MORITA Yuhei

出版者

Japanese Society of Plant Physiologists

雑誌

Plant and cell physiology (ISSN:00320781)

巻号頁・発行日

vol.35, no.7, pp.1049-1056, 1994-10

参考文献数

38

被引用文献数

6

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A proteolytic activity directed against the a subunit of β-conglycinin was detected in resting mature seeds of the soybean [Glycine max (L.) Merrill] cultivar Keburi. The relationship between pH and activity and the effect of protease inhibitors revealed that the enzyme was a neutral/alkaline serine protease. The proteolysis of the a subunit of β-conglycinin yielded a specific product with a molecular weight of about 47,000, as determined by SDS-PAGE, but the enzyme had no activity against the β subunit. The amino acid composition, the molecular weight and the amino-terminal amino acid sequence of the proteolytic product revealed that the action of the enzyme on the a subunit was specific, with cleavage occurring only at the R126-R127 peptide bond of the a subunit. These characteristics of the protease indicate that the enzyme is a novel protease that has not previously been recognized in soybean seeds.

著者

Kasahara Masahiro Yashiro Kumiko Sakamoto Toshio Ohmori Masayuki

出版者

Japanese Society of Plant Physiologists

雑誌

Plant and cell physiology (ISSN:00320781)

巻号頁・発行日

vol.38, no.7, pp.828-836, 1997

参考文献数

48

被引用文献数

3

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A cyaC gene encoding an adenylate cyclase of the filamentous cyanobacterium Spirulina platensis was sequenced. The predicted amino acid sequence of the C-terminal region of cyaC is similar to the catalytic domains of adenylate cyclases in other cyanobacteria and eukaryotes. The sequences of other regions are similar to those of proteins consisting of the bacterial two-component signal transduction system: the sensory kinase and the response regulator. The predicted gene product of cyaC contains, from the N-terminal end, a receiver domain of the response regulator protein (R1), a domain similar to the ETR1 of Arabidopsis thaliana, a transmitter domain of the sensory kinase protein, a receiver domain of the response regulator protein (R2), and a catalytic domain of adenylate cyclase. The cyaC gene was expressed as an affinity-tagged protein in Escherichia coli, and the recombinant protein was purified. The purified protein had adenylate cyclase activity which was activated by Mn^<2+>. The results of Western blotting using an anti-CyaC antiserum and the S. platensis cell extract confirmed that cyaC gene is expressed in S. platensis.

著者

Eklof Staffan Astot Crister Blackwell John Thomas Moritz Olof Olsson Goran Sandberg Department of Forest Genetics and Plant physiology SwedishUniversity of Agricltural sciences Department of Forest Genetics and Plant physiology SwedishUniversity of Agricltural sciences Department of Forest Genetics and Plant physiology SwedishUniversity of Agricltural sciences Department of Forest Genetics and Plant physiology SwedishUniversity of Agricltural sciences Department of Molecular Biology Goteborg University Department of Forest Genetics and Plant physiology SwedishUniversity of Agricltural sciences

出版者

Japanese Society of Plant Physiologists

雑誌

Plant and cell physiology (ISSN:00320781)

巻号頁・発行日

vol.38, no.3, pp.225-235, 1997-03

参考文献数

59

被引用文献数

3

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Cytokinins and auxins are important regulators of plant growth and development, but there is incomplete and conflicting evidence that auxins affect cytokinin metabolism and vice versa. We have investigated these interactions in Nicotiana tabacum L. by separate in planta manipulation of levels of the hormones followed by analysis of the induced changes in the metabolism of the other hormone. Cytokinin-overproducing plants (expressing the Agrobacterium tumefaciens ipt gene) had lower than wild-type levels of free IAA, and reduced rates of IAA synthesis and turn-over, but there were no differences in the profiles of metabolites they produced from fed IAA. Similarly, auxin-over-producing plants (expressing the A. tumefaciens iaaM and iaaH genes), had lower levels of the major cytokinins than wild-type plants and lower cytokinin oxidase activity, but there were no differences in the profiles of metabolites they produced from fed cytokinins. The data demonstrate that cytokinin or auxin overproduction decreases the content of the other hormone, apparently by decreasing its rate of synthesis and/or transport, rather than by increasing rates of turnover or conjugation. Implications for the importance of cytokinin : auxin ratios in plant development are considered.