- 著者
-
Yasumoto Shuhei
Umemoto Naoyuki
Lee Hyoung Jae
Nakayasu Masaru
Sawai Satoru
Sakuma Tetsushi
Yamamoto Takashi
Mizutani Masaharu
Saito Kazuki
Muranaka Toshiya
- 出版者
- 日本植物細胞分子生物学会
- 雑誌
- Plant Biotechnology (ISSN:13424580)
- 巻号頁・発行日
- vol.36, no.3, pp.167-173, 2019
- 被引用文献数
-
32
<p>Potato (<i>Solanum tuberosum</i>) is one of the most important crops in the world. However, it is generally difficult to breed a new variety of potato crops because they are highly heterozygous tetraploid. Steroidal glycoalkaloids (SGAs) such as α-solanine and α-chaconine found in potato are antinutritional specialized metabolites. Because of their toxicity following intake, controlling the SGA levels in potato varieties is critical in breeding programs. Recently, genome-editing technologies using artificial site-specific nucleases such as TALEN and CRISPR-Cas9 have been developed and used in plant sciences. In the present study, we developed a highly active Platinum TALEN expression vector construction system, and applied to reduce the SGA contents in potato. Using <i>Agrobacterium-</i>mediated transformation, we obtained three independent transgenic potatoes harboring the TALEN expression cassette targeting SSR2 gene, which encodes a key enzyme for SGA biosynthesis. Sequencing analysis of the target sequence indicated that all the transformants could be <i>SSR2</i>-knockout mutants. Reduced SGA phenotype in the mutants was confirmed by metabolic analysis using LC-MS. In vitro grown <i>SSR2</i>-knockout mutants exhibited no differences in morphological phenotype or yields when compared with control plants, indicating that the genome editing of SGA biosynthetic genes such as <i>SSR2</i> could be a suitable strategy for controlling the levels of toxic metabolites in potato. Our simple and powerful plant genome-editing system, developed in the present study, provides an important step for future study in plant science.</p>