著者

Kaneko Takehito Sakuma Tetsushi Yamamoto Takashi Mashimo Tomoji

出版者

Nature Publishing Group

雑誌

Scientific reports (ISSN:20452322)

巻号頁・発行日

vol.4, 2014-10-01

被引用文献数

173

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超簡単!遺伝子改変動物作製法の開発 -エレクトロポレーション(電気穿孔)法による哺乳類受精卵への ZFN、TALEN、CRISPR-Casの導入に成功-. 京都大学プレスリリース. 2014-10-03.

著者

Mashimo Tomoji Kaneko Takehito Sakuma Tetsushi Kobayashi Junya Kunihiro Yayoi Voigt Birger Yamamoto Takashi Serikawa Tadao

出版者

Nature Publishing Group

雑誌

Scientific Reports

巻号頁・発行日

vol.3, 2013-02-13

被引用文献数

71

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TALエフェクターヌクレアーゼ(TALEN)を用いた効率的な遺伝子改変ラットの作製技術. 京都大学プレスリリース. 2013-02-13.

著者

Yasumoto Shuhei Umemoto Naoyuki Lee Hyoung Jae Nakayasu Masaru Sawai Satoru Sakuma Tetsushi Yamamoto Takashi Mizutani Masaharu Saito Kazuki Muranaka Toshiya

出版者

日本植物細胞分子生物学会

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.36, no.3, pp.167-173, 2019

被引用文献数

32

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<p>Potato (<i>Solanum tuberosum</i>) is one of the most important crops in the world. However, it is generally difficult to breed a new variety of potato crops because they are highly heterozygous tetraploid. Steroidal glycoalkaloids (SGAs) such as α-solanine and α-chaconine found in potato are antinutritional specialized metabolites. Because of their toxicity following intake, controlling the SGA levels in potato varieties is critical in breeding programs. Recently, genome-editing technologies using artificial site-specific nucleases such as TALEN and CRISPR-Cas9 have been developed and used in plant sciences. In the present study, we developed a highly active Platinum TALEN expression vector construction system, and applied to reduce the SGA contents in potato. Using <i>Agrobacterium-</i>mediated transformation, we obtained three independent transgenic potatoes harboring the TALEN expression cassette targeting SSR2 gene, which encodes a key enzyme for SGA biosynthesis. Sequencing analysis of the target sequence indicated that all the transformants could be <i>SSR2</i>-knockout mutants. Reduced SGA phenotype in the mutants was confirmed by metabolic analysis using LC-MS. In vitro grown <i>SSR2</i>-knockout mutants exhibited no differences in morphological phenotype or yields when compared with control plants, indicating that the genome editing of SGA biosynthetic genes such as <i>SSR2</i> could be a suitable strategy for controlling the levels of toxic metabolites in potato. Our simple and powerful plant genome-editing system, developed in the present study, provides an important step for future study in plant science.</p>

著者

Ninagawa Satoshi Okada Tetsuya Sumitomo Yoshiki Kamiya Yukiko Kato Koichi Horimoto Satoshi Ishikawa Tokiro Takeda Shunichi Sakuma Tetsushi Yamamoto Takashi Mori Kazutoshi

出版者

Rockefeller University Press

雑誌

The Journal of cell biology (ISSN:15408140)

巻号頁・発行日

vol.206, no.3, pp.347-356, 2014-08-04

被引用文献数

126

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構造異常糖タンパク質の分解に必須な糖鎖刈り込み機構を解明 -最新ゲノム編集解析により醜いアヒルの子が美しい白鳥に変身-. 京都大学プレスリリース. 2014-09-02.