著者
庫本 高志 横江 繭子 矢ヶ崎 佳代子 KAWAGUCHI Tatsuya KUMAFUJI Kenta SERIKAWA Tadao
出版者
Japanese Association for Laboratory Animal Science
雑誌
Experimental Animals (ISSN:13411357)
巻号頁・発行日
vol.59, no.2, pp.147-155, 2010
被引用文献数
1 22

To collect rat mutations and increase the value of the rat model system, we introduced fancy-derived mutations to the laboratory and carried out genetic analyses. Six fancy rats were shipped from a fancy rat colony in the USA and used as founders. After initial crosses with a laboratory strain, TM/Kyo or PVG/Seac, inbreeding started and 6 partially inbred lines, including 2 sublines, were produced as Kyoto Fancy Rat Stock (KFRS) strains. During inbreeding, we isolated 9 mutations: 5 coat colors, American mink (<i>am</i>), Black eye (<i>Be</i>), grey (<i>g</i>), Pearl (<i>Pel</i>), siamese (<i>sia</i>); 1 coat pattern, head spot (<i>hs</i>); 2 coat textures, Rex (<i>Re</i>), satin (<i>sat</i>); and an ear pinnae malformation, dumbo (<i>dmbo</i>). Genetic analyses mapped 7 mutations to particular regions of the rat chromosomes (Chr): <i>am</i> to Chr 1, <i>sia</i> to Chr 1, <i>sat</i> to Chr 3, <i>Re</i> to Chr 7, <i>g</i> to Chr 8, <i>dmbo</i> to Chr 14, and <i>hs</i> to Chr 15. Candidate gene analysis revealed that a missense mutation in the tyrosinase gene, Ser79Pro, was responsible for <i>sia</i>. From mutant phenotypes and mapping positions, it is likely that all mutations isolated in this study were unique to the fancy rat. These findings suggest that fancy rat colonies are a good source for collecting rat mutations. The fancy-derived mutations, made available to biomedical research in the current study, will increase the scientific value of laboratory rats.<br>
著者
Mashimo Tomoji Kaneko Takehito Sakuma Tetsushi Kobayashi Junya Kunihiro Yayoi Voigt Birger Yamamoto Takashi Serikawa Tadao
出版者
Nature Publishing Group
雑誌
Scientific Reports
巻号頁・発行日
vol.3, 2013-02-13
被引用文献数
71

TALエフェクターヌクレアーゼ(TALEN)を用いた効率的な遺伝子改変ラットの作製技術. 京都大学プレスリリース. 2013-02-13.
著者
Mashimo Tomoji Takizawa Akiko Kobayashi Junya Kunihiro Yayoi Yoshimi Kazuto Ishida Saeko Tanabe Koji Yanagi Ami Tachibana Asato Hirose Jun Yomoda Jun-Ichiro Morimoto Shiho Kuramoto Takashi Voigt Birger Watanabe Takeshi Hiai Hiroshi Tateno Chise Komatsu Kenshi Serikawa Tadao
出版者
Elsevier
雑誌
Cell reports (ISSN:22111247)
巻号頁・発行日
vol.2, no.3, pp.685-694, 2012-09-27
被引用文献数
60

重症免疫不全SCIDラットの作製 : ヒトiPS細胞、がん細胞、肝細胞をラット体内で培養することに成功. 京都大学プレスリリース. 2012-09-14.